Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses

Qi, Furong1; Qian, Shen1; Zhang, Shuye3,4; Zhang, Zheng1,2

2020-05-21

10.1016/j.bbrc.2020.03.044

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Impact Factor & Quartile

0006-291X

1090-2104

The new coronavirus (SARS-CoV-2) outbreak from December 2019 in Wuhan, Hubei, China, has been declared a global public health emergency. Angiotensin I converting enzyme 2 (ACE2), is the host receptor by SARS-CoV-2 to infect human cells. Although ACE2 is reported to be expressed in lung, liver, stomach, ileum, kidney and colon, its expressing levels are rather low, especially in the lung. SARS-CoV-2 may use co-receptors/auxiliary proteins as ACE2 partner to facilitate the virus entry. To identify the potential candidates, we explored the single cell gene expression atlas including 119 cell types of 13 human tissues and analyzed the single cell co-expression spectrum of 51 reported RNA virus receptors and 400 other membrane proteins. Consistent with other recent reports, we confirmed that ACE2 was mainly expressed in lung AT2, liver cholangiocyte, colon colonocytes, esophagus keratinocytes, ileum ECs, rectum ECs, stomach epithelial cells, and kidney proximal tubules. Intriguingly, we found that the candidate co-receptors, manifesting the most similar expression patterns with ACE2 across 13 human tissues, are all peptidases, including ANPEP, DPP4 and ENPEP. Among them, ANPEP and DPP4 are the known receptors for human CoVs, suggesting ENPEP as another potential receptor for human CoVs. We also conducted "CellPhoneDB" analysis to understand the cell crosstalk between Coy-targets and their surrounding cells across different tissues. We found that macrophages frequently communicate with the CoVs targets through chemokine and phagocytosis signaling, highlighting the importance of tissue macrophages in immune defense and immune pathogenesis. (C) 2020 Shenzhen Third People\u0019s Hospital. Published by Elsevier Inc.

Coronaviruses SARS-CoV-2 scRNA-seq ACE2 Co-receptor Macrophage

SCI

English

ESI Highly Cited Papers ; ESI Hot Papers

Corresponding

Biochemistry & Molecular Biology ; Biophysics

Biochemistry & Molecular Biology ; Biophysics

WOS:000530586500021

ACADEMIC PRESS INC ELSEVIER SCIENCE

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Shenzhen Third Peoples Hosp, Natl Clin Res Ctr Infect Dis, Inst Hepatol, Shenzhen 518100, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 2, Shenzhen 518112, Guangdong, Peoples R China
3.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China
4.Fudan Univ, Inst Biomed Sci, Shanghai, Peoples R China

Qi, Furong,Qian, Shen,Zhang, Shuye,et al. Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2020,526(1):135-140.

Qi, Furong,Qian, Shen,Zhang, Shuye,&Zhang, Zheng.(2020).Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,526(1),135-140.

Qi, Furong,et al."Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 526.1(2020):135-140.