中文版 | English
Title

Lnc-OC1在卵巢癌和乳腺癌中的功能探究

Alternative Title
ROLE OF LNC-OC1 IN OVARIAN AND BREAST CANCER CELLS
Author
Name pinyin
DENG Limin
School number
11930136
Degree
硕士
Discipline
0710 生物学
Subject category of dissertation
07 理学
Supervisor
董金堂
Mentor unit
人类细胞生物和遗传学系
Publication Years
2022-04-27
Submission date
2022-06-27
University
南方科技大学
Place of Publication
深圳
Abstract

卵巢癌和乳腺癌是两种严重危害女性健康的恶性肿瘤,对病人和社会经济都造成了巨大的负担。我国由于人口基数大,卵巢癌和乳腺癌的发病人数和死亡人数都高居世界前列,对其更为深入的研究将有助于相关肿瘤病人的诊断、治疗和预后。已有报道证明长链非编码RNALncRNA)在多种癌症中扮演着重要作用,我们的前期研究发现了一个在卵巢癌中具有功能的新的lncRNA,即LOC100288181,并将其命名为Lnc-OC1。在卵巢癌中,Lnc-OC1通过吸附miR-34a/miR-34c并抑制后者功能来进而促进卵巢癌细胞的增殖和迁移。本课题从一例中国女性卵巢癌病人腹水样本中分离并进行培养,得到原代培养细胞,通过免疫荧光、细胞流式分析鉴定其为癌细胞,并命名为Sample1在此基础上进行了体外和体内一系列功能探究。结果表明,敲减Lnc-OC1显著抑制了Sample1细胞的增殖、迁移和浸润,促进了细胞凋亡。裸鼠体内成瘤实验表明,敲减Lnc-OC1显著抑制了肿瘤生长。耐药实验提示,卵巢癌中Lnc-OC1的表达和卡铂以及紫杉醇的耐药没有明显关系。我们也对Lnc-OC1在乳腺癌中的作用进行了初步探究。在乳腺癌细胞系MDA-MB-231进行Lnc-OC1敲减处理,发现细胞增殖、细胞成球能力和干细胞比例均受到抑制。上述结果表明,Lnc-OC1在卵巢癌和乳腺癌的发生过程中扮演着癌基因的角色,有可能成为一种潜在的诊断生物标志物和治疗靶点。

Other Abstract

Ovarian cancer and breast cancer represent two kinds of malignant tumors which seriously threat women's health and bring a huge burden to the patients. Due to the large population in China, the patient numbers and deaths of ovarian and breast cancers are among the highest in the world. In-depth study of molecular mechanisms underlying ovarian and breast tumor development is helpful to the diagnosis, treatment, and prognosis of relevant tumor patients. It has been reported that long non-coding RNAs lncRNAs play an extremely important role in a wide-range of cancers. Our lab previously reported a novel LncRNA LOC100288181, named as Lnc-OC1 in ovarian cancer. Lnc-OC1 promotes the proliferation and migration of ovarian cancer cells by sponging miR-34a/miR-34c. In this study, a primary culture named Sample1 was established from patient ascites and was used to test Lnc-OC1 function. Immunofluorescence staining and flow cytometry analysis demonstrated that the primary culture had the properties of epithelial cancer cells. A series of functional in vitro and in vivo experiments demonstrated that knockdown of Lnc-OC1 significantly inhibited cell proliferation, cell migration, and cell invasion, while promoting apoptosis. Tumorigenesis experiment showed that knockdown of Lnc-OC1 inhibited tumor growth. In addition, preliminary study suggests that Lnc-OC1 is not involved in the resistance of ovarian cancer cells to carboplatin or paclitaxel. In breast cancer cell line MDA-MB-231, knockdown of Lnc-OC1 could suppress the cell proliferation, cell spheroid capacity and stem cell ratio. These results suggest that Lnc-OC1 promotes the growth of ovarian and breast cancers, playing an oncogenic role.

Keywords
Language
Chinese
Training classes
独立培养
Enrollment Year
2019
Year of Degree Awarded
2022-07
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Academic Degree Assessment Sub committee
医学院
Domestic book classification number
R73-3
Data Source
人工提交
Document TypeThesis
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/343018
DepartmentSchool of Medicine
Recommended Citation
GB/T 7714
邓丽敏. Lnc-OC1在卵巢癌和乳腺癌中的功能探究[D]. 深圳. 南方科技大学,2022.
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