| Title | Deformable mirror based optimal PSF engineering for 3D super-resolution imaging |
| Author | |
| Corresponding Author | Li, Yiming |
| Publication Years | 2022-06-15
|
| DOI | |
| Source Title | |
| ISSN | 0146-9592
|
| EISSN | 1539-4794
|
| Volume | 47Issue:12Pages:3031-3034 |
| Abstract | Point spread function (PSF) engineering is an important technique to encode the properties (e.g., 3D positions, color, and orientation) of a single molecule in the shape of the PSF, often with the help of a programmable phase modulator. A deformable mirror (DM) is currently the most widely used phase modulator for fluorescence detection as it shows negligible photon loss. However, it relies on careful calibration for precise wavefront control. Therefore, design of an optimal PSF not only relies on the theoretical calculation of the maximum information content, but also the physical behavior of the phase modulator, which is often ignored during the optimization process. Here, we develop a framework for PSF engineering which could generate a device specific optimal PSF for 3D super-resolution imaging using a DM. We use our method to generate two types of PSFs with depths of field comparable to the widely used astigmatism and tetrapod PSFs, respectively. We demonstrate the superior performance of the DM specific optimal PSF over the conventional astigmatism and tetrapod PSF both theoretically and experimentally. (C) 2022 Optica Publishing Group |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | First
; Corresponding
|
| Funding Project | Shandong Key Research and Development Program[2021CXGC010212]
; Science, Technology and Innovation Commission of Shenzhen Municipality[KQTD20200820113012029]
; Guangdong Natural Science Foundation[2020A1515110380]
; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003]
|
| WOS Research Area | Optics
|
| WOS Subject | Optics
|
| WOS Accession No | WOS:000812307400024
|
| Publisher | |
| EI Accession Number | 20222512261759
|
| EI Keywords | Adaptive optics
; Deformation
; Delta modulation
; Mirrors
; Optical resolving power
; Phase modulation
|
| ESI Classification Code | Light/Optics:741.1
; Optical Devices and Systems:741.3
|
| ESI Research Field | PHYSICS
|
| Data Source | Web of Science
|
| Citation statistics |
Cited Times [WOS]:3
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/343023 |
| Department | Department of Biomedical Engineering |
| Affiliation | 1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China 2.Southern Univ Sci & Technol, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Peoples R China 3.Zhejiang Univ, State Key Lab Modern Opt Instrumentat, Coll Opt Sci & Technol, Hangzhou 310027, Peoples R China |
| First Author Affilication | Department of Biomedical Engineering; Southern University of Science and Technology |
| Corresponding Author Affilication | Department of Biomedical Engineering; Southern University of Science and Technology |
| First Author's First Affilication | Department of Biomedical Engineering |
| Recommended Citation GB/T 7714 |
Fu, Shuang,Li, Mengfan,Zhou, Lulu,et al. Deformable mirror based optimal PSF engineering for 3D super-resolution imaging[J]. OPTICS LETTERS,2022,47(12):3031-3034.
|
| APA |
Fu, Shuang.,Li, Mengfan.,Zhou, Lulu.,He, Yingchuan.,Liu, Xin.,...&Li, Yiming.(2022).Deformable mirror based optimal PSF engineering for 3D super-resolution imaging.OPTICS LETTERS,47(12),3031-3034.
|
| MLA |
Fu, Shuang,et al."Deformable mirror based optimal PSF engineering for 3D super-resolution imaging".OPTICS LETTERS 47.12(2022):3031-3034.
|
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