Three-Dimensional Printing Self-Healing Dynamic/Photocrosslinking Gelatin-Hyaluronic Acid Double-Network Hydrogel for Tissue Engineering

Wang, Yunping1; Chen, Yazhen1; Zheng, Jianuo1; Liu, Lingrong1; Zhang, Qiqing1,2,3

2022-04-12

10.1021/acsomega.2c00335

ACS Omega   Impact Factor & Quartile

2470-1343

Three-dimensional (3D) printing technology has great potential for constructing structurally and functionally complex scaffold materials for tissue engineering. Bio-inks are a critical part of 3D printing for this purpose. In this study, based on dynamic hydrazone-crosslinked hyaluronic acid (HA-HYD) and photocrosslinked gelatin methacrylate (GeIMA), a double-network (DN) hydrogel with significantly enhanced mechanical strength, self-healing, and shear-thinning properties was developed as a printable hydrogel bio-ink for extrusion-based 3D printing. Owing to shear thinning, the DN hydrogel bio-inks could be extruded to form uniform filaments, which were printed layer by layer to fabricate the scaffolds. The self-healing performance of the filaments and photocrosslinking of GeIMA worked together to obtain an integrated and stable printed structure with high mechanical strength. The in vitro cytocompatibility assay showed that the DN hydrogel printed scaffolds supported the survival and proliferation of bone marrow mesenchymal stem cells. GeIMA/HA-HYD DN hydrogel bio-inks with printability, good structural integrity, and biocompatibility are promising materials for 3D printing of tissue engineering scaffolds.

SCI

English

Others

National Key Basic Research and Development Projects[2017YFC1103601] ; Key Project of Guangdong Basic and Applied Basic Research Foundation[2020B1515120091]

Chemistry

Chemistry, Multidisciplinary

WOS:000812923300001

AMER CHEMICAL SOC

Web of Science

1.Chinese Acad Med Sci & Peking Union Med Coll, Inst Biomed Engn, Tianjin Key Lab Biomed Mat, Tianjin 300192, Peoples R China
2.South Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Inst Biomed Engn, Shenzhen 518020, Guangdong, Peoples R China
3.Fujian Bote Biotechnol Co Ltd, Fuzhou 350013, Fujian, Peoples R China

Wang, Yunping,Chen, Yazhen,Zheng, Jianuo,et al. Three-Dimensional Printing Self-Healing Dynamic/Photocrosslinking Gelatin-Hyaluronic Acid Double-Network Hydrogel for Tissue Engineering[J]. ACS Omega,2022,7(14).

Wang, Yunping,Chen, Yazhen,Zheng, Jianuo,Liu, Lingrong,&Zhang, Qiqing.(2022).Three-Dimensional Printing Self-Healing Dynamic/Photocrosslinking Gelatin-Hyaluronic Acid Double-Network Hydrogel for Tissue Engineering.ACS Omega,7(14).

Wang, Yunping,et al."Three-Dimensional Printing Self-Healing Dynamic/Photocrosslinking Gelatin-Hyaluronic Acid Double-Network Hydrogel for Tissue Engineering".ACS Omega 7.14(2022).