| Title | Global fitting for high-accuracy multi-channel single-molecule localization |
| Author | |
| Corresponding Author | Li, Yiming; Ries, Jonas |
| Publication Years | 2022-06-06
|
| DOI | |
| Source Title | |
| EISSN | 2041-1723
|
| Volume | 13Issue:1 |
| Abstract | ["Multi-channel SMLM imaging is powerful. Here the authors report globLoc, a GPU-based global fitting algorithm, to extract maximum information from multichannel single molecule data; this gives improved localisation precision for biplane and 4Pi-SMLM and colour assignment in multi-colour astigmatic SMLM.","Multi-channel detection in single-molecule localization microscopy greatly increases information content for various biological applications. Here, we present globLoc, a graphics processing unit based global fitting algorithm with flexible PSF modeling and parameter sharing, to extract maximum information from multi-channel single molecule data. As signals in multi-channel data are highly correlated, globLoc links parameters such as 3D coordinates or photon counts across channels, improving localization precision and robustness. We show, both in simulations and experiments, that global fitting can substantially improve the 3D localization precision for biplane and 4Pi single-molecule localization microscopy and color assignment for ratiometric multicolor imaging."] |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| Important Publications | NI Journal Papers
|
| SUSTech Authorship | First
; Corresponding
|
| Funding Project | Key Technology Research and Development Program of Shandong[2021CXGC010212]
; Guangdong Natural Science Foundation Joint Fund[2020A1515110380]
; Shenzhen Science and Technology Innovation Commission[KQTD20200820113012029]
; European Research Council[ERC CoG-724489]
; National Institutes of Health Common Fund 4D Nucleome Program[U01 EB021223]
; Human Frontier Science Program[RGY0065/2017]
|
| WOS Research Area | Science & Technology - Other Topics
|
| WOS Subject | Multidisciplinary Sciences
|
| WOS Accession No | WOS:000808000200008
|
| Publisher | |
| Scopus EID | 2-s2.0-85131323320
|
| Data Source | Web of Science
|
| Citation statistics |
Cited Times [WOS]:7
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/343092 |
| Department | Department of Biomedical Engineering |
| Affiliation | 1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China 2.European Mol Biol Lab Cell Biol & Biophys, D-69117 Heidelberg, Germany 3.Fac Biosci, Collaborat Joint PhD Degree EMBL & Heidelberg Uni, Heidelberg, Germany |
| First Author Affilication | Department of Biomedical Engineering |
| Corresponding Author Affilication | Department of Biomedical Engineering |
| First Author's First Affilication | Department of Biomedical Engineering |
| Recommended Citation GB/T 7714 |
Li, Yiming,Shi, Wei,Liu, Sheng,et al. Global fitting for high-accuracy multi-channel single-molecule localization[J]. NATURE COMMUNICATIONS,2022,13(1).
|
| APA |
Li, Yiming.,Shi, Wei.,Liu, Sheng.,Cavka, Ivana.,Wu, Yu-Le.,...&Ries, Jonas.(2022).Global fitting for high-accuracy multi-channel single-molecule localization.NATURE COMMUNICATIONS,13(1).
|
| MLA |
Li, Yiming,et al."Global fitting for high-accuracy multi-channel single-molecule localization".NATURE COMMUNICATIONS 13.1(2022).
|
| Files in This Item: | There are no files associated with this item. | |||||
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