时间分辨荧光免疫分析新冠抗原检测

新冠病毒肆虐全球，做好新冠病毒抗原的检测，对疫情防控具有重要意义。免疫分析法是一类检测抗原的便捷方法，研究和推广应用免疫分析法是提高病毒抗原检测质效的便捷途径。免疫分析法可分为层析法和微孔板法。在诸多的免疫分析检测标记中，镧系金属配合物掺杂的荧光微球有着无可比拟的优势，发射荧光寿命长、不受激发光干扰等。本文通过层析法和微孔板法，对时间分辨免疫分析检测新冠抗原进行了研究。基于产品应用的考虑，以新冠抗原为检测对象，制备了以时间分辨荧光微球偶联新冠抗体为探针的试纸条，从定性和定量两个方面对试纸条检测新冠抗原的性能进行了分析，试纸条检测抗原的检测限为0.86ng/mL。为实现对新冠病毒两种不同抗原的同时检测，在已有的铕微球探针基础上，通过实验验证和对比分析，制备合成了掺杂铽配合物的二氧化硅微球，并实现了表面羧基化。利用合成的铽微球制备成探针，联合已有的铕微球探针使用微孔板作为检测的支撑环境，实现了在同一微孔环境中，用两种时间分辨荧光微球探针同时检测混合有两种抗原的样本。两种探针均采用同样的230nm波长激发光，铽微球探针检测新冠抗原的LOD并未受到铕微球探针的影响，实现了两种抗原在同一条件下独立检测，检测N蛋白抗原和S蛋白抗原的LOD分别为75pg/mL、82pg/mL。

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