中文版 | English
Title

Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics

Author
Corresponding AuthorLi,Pengfei; Wang,Fangjun; Tian,Ruijun
Publication Years
2022-06-16
DOI
Source Title
ISSN
2451-9456
EISSN
2451-9448
Volume29Issue:6Pages:1024-1036
Abstract

Identification of the tyrosine phosphorylation (pY)-dependent interactome of immune co-receptors is crucial for understanding signal pathways involved in immunotherapy. However, identifying the motif-specific interactome for each pY commonly found on these multi-phosphorylated membrane proteins remains challenging. Here, we describe a photoaffinity-based chemical proteomic approach to dissect the motif-specific cytoplasmic interactomes of the critical immune co-receptor CD28. Various full-length CD28 cytoplasmic tails (CD28(cyto)) with defined pY and selectively replaced photo-methionine were synthesized and applied to explore three pY-motif-dependent CD28(cyto) interactomes. We identified a stand-alone interaction of phospholipase PLCG1 with the Y191 motif with enhanced affinity for the sequence neighboring the transmembrane domain. Importantly, taking advantage of native top-down mass spectrometry with a 193-nm laser, we discovered the direct association of a previously undefined pY218 motif with the kinase PKCq through its C2 domain. This synthetic CD28(cyto)-based photoaffinity proteomic approach is generically applicable to the study of other immune co-receptors with multiple pY sites on their linear cytoplasmic tail.

URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
First ; Corresponding
Funding Project
China State Key Basic Research Program[2020YFE0202200] ; Original Innovation Project of CAS[ZDBS-LY-SLH032] ; National Natural Science Foundation of China[
WOS Research Area
Biochemistry & Molecular Biology
WOS Subject
Biochemistry & Molecular Biology
WOS Accession No
WOS:000829325000010
Publisher
Scopus EID
2-s2.0-85132454219
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:3
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/343321
DepartmentDepartment of Chemistry
理学院
南方科技大学医学院
冷冻电镜中心
Affiliation
1.Department of Chemistry,College of Science,Southern University of Science and Technology,Shenzhen,518055,China
2.CAS Key Laboratory of Separation Sciences for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,116023,China
3.State Key Laboratory of Molecular Reaction Dynamics,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,116023,China
4.University of Chinese Academy of Sciences,Beijing,100049,China
5.Cryo-EM Center,Southern University of Science and Technology,Shenzhen,518055,China
6.Synthetic and Functional Biomolecules Center,Beijing National Laboratory for Molecular Sciences,College of Chemistry and Molecular Engineering,Peking University,Beijing,China
7.Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research,Southern University of Science and Technology,Shenzhen,518055,China
First Author AffilicationDepartment of Chemistry;  College of Science
Corresponding Author AffilicationDepartment of Chemistry;  College of Science;  School of Medicine
First Author's First AffilicationDepartment of Chemistry;  College of Science
Recommended Citation
GB/T 7714
Chen,Xiong,Ji,Shanping,Liu,Zheyi,et al. Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics[J]. CELL CHEMICAL BIOLOGY,2022,29(6):1024-1036.
APA
Chen,Xiong.,Ji,Shanping.,Liu,Zheyi.,Yuan,Xiao.,Xu,Congsheng.,...&Tian,Ruijun.(2022).Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics.CELL CHEMICAL BIOLOGY,29(6),1024-1036.
MLA
Chen,Xiong,et al."Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics".CELL CHEMICAL BIOLOGY 29.6(2022):1024-1036.
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