Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics
|Corresponding Author||Li，Pengfei; Wang，Fangjun; Tian，Ruijun|
Identification of the tyrosine phosphorylation (pY)-dependent interactome of immune co-receptors is crucial for understanding signal pathways involved in immunotherapy. However, identifying the motif-specific interactome for each pY commonly found on these multi-phosphorylated membrane proteins remains challenging. Here, we describe a photoaffinity-based chemical proteomic approach to dissect the motif-specific cytoplasmic interactomes of the critical immune co-receptor CD28. Various full-length CD28 cytoplasmic tails (CD28(cyto)) with defined pY and selectively replaced photo-methionine were synthesized and applied to explore three pY-motif-dependent CD28(cyto) interactomes. We identified a stand-alone interaction of phospholipase PLCG1 with the Y191 motif with enhanced affinity for the sequence neighboring the transmembrane domain. Importantly, taking advantage of native top-down mass spectrometry with a 193-nm laser, we discovered the direct association of a previously undefined pY218 motif with the kinase PKCq through its C2 domain. This synthetic CD28(cyto)-based photoaffinity proteomic approach is generically applicable to the study of other immune co-receptors with multiple pY sites on their linear cytoplasmic tail.
First ; Corresponding
China State Key Basic Research Program[2020YFE0202200] ; Original Innovation Project of CAS[ZDBS-LY-SLH032] ; National Natural Science Foundation of China[
|WOS Research Area|
Biochemistry & Molecular Biology
Biochemistry & Molecular Biology
|WOS Accession No|
Web of Science
Cited Times [WOS]:3
|Document Type||Journal Article|
|Department||Department of Chemistry|
1.Department of Chemistry,College of Science,Southern University of Science and Technology,Shenzhen,518055,China
2.CAS Key Laboratory of Separation Sciences for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,116023,China
3.State Key Laboratory of Molecular Reaction Dynamics,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,116023,China
4.University of Chinese Academy of Sciences,Beijing,100049,China
5.Cryo-EM Center,Southern University of Science and Technology,Shenzhen,518055,China
6.Synthetic and Functional Biomolecules Center,Beijing National Laboratory for Molecular Sciences,College of Chemistry and Molecular Engineering,Peking University,Beijing,China
7.Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research,Southern University of Science and Technology,Shenzhen,518055,China
|First Author Affilication||Department of Chemistry; College of Science|
|Corresponding Author Affilication||Department of Chemistry; College of Science; School of Medicine|
|First Author's First Affilication||Department of Chemistry; College of Science|
Chen，Xiong,Ji，Shanping,Liu，Zheyi,et al. Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics[J]. CELL CHEMICAL BIOLOGY,2022,29(6):1024-1036.
Chen，Xiong.,Ji，Shanping.,Liu，Zheyi.,Yuan，Xiao.,Xu，Congsheng.,...&Tian，Ruijun.(2022).Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics.CELL CHEMICAL BIOLOGY,29(6),1024-1036.
Chen，Xiong,et al."Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics".CELL CHEMICAL BIOLOGY 29.6(2022):1024-1036.
|Files in This Item:||There are no files associated with this item.|
|Recommend this item|
|Export to Endnote|
|Export to Excel|
|Export to Csv|
|Similar articles in Google Scholar|
|Similar articles in Baidu Scholar|
|Similar articles in Bing Scholar|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.