In situ forming injectable gamma-poly(glutamic acid)/PEG adhesive hydrogels for hemorrhage control

Ye, Huijun; Xian, Yiwen; Li, Shurong; Zhang, Chong; Wu, Decheng

2022-06-01

10.1039/d2bm00525e

Biomaterials Science   Impact Factor & Quartile

2047-4830

2047-4849

Rapidly in situ forming adhesive hydrogels are promising candidates for efficient hemostasis due to their easy administration and minimal invasion. However, development of biocompatible and high-performance hemostatic hydrogels without any additional toxic agents remains a challenge. Herein, a series of novel injectable adhesive hydrogels based on N-hydroxysuccinimide (NHS) modified gamma-poly(glutamic acid) (gamma PGA-NHS) and tetra-armed poly(ethylene glycol) amine (Tetra-PEG-NH2) were developed. Among all samples, PGA(10)-PEG(15) and PGA(10)-PEG(20) hydrogels with higher PEG contents exhibited rapid gelation time (<20 s), strong mechanical strength (compression modulus up to similar to 75 kPa), good adhesive properties (similar to 15 kPa), and satisfactory burst pressure (similar to 18-20 kPa). As a result, PGA(10)-PEG(15) and PGA(10)-PEG(20) hydrogels showed a remarkable reduction in hemostasis time and blood loss compared with gauze and fibrin glue. More importantly, the PGA(10)-PEG(20) hydrogel was also successfully used to seal femoral arterial trauma. Subcutaneous implantation experiments indicated a good biocompatibility of the hydrogels in vivo. All these results strongly support that the developed PGA-PEG hydrogels could serve as promising hemostatic agents in emergency and clinical situations.

SCI ; EI

English

First ; Corresponding

Ministry of Science and Technology of China[2020YFA0908900] ; National Natural Science Foundation of China[21935011,21725403] ; Shenzhen Science and Technology Innovation Commission[KQTD20200820113012029]

Materials Science

Materials Science, Biomaterials

WOS:000815215600001

ROYAL SOC CHEMISTRY

20222712305945

Adhesives ; Amino acids ; Biocompatibility ; Gelation ; Hydrogels

Immunology:461.9.1 ; Colloid Chemistry:801.3 ; Chemical Operations:802.3 ; Chemical Products Generally:804 ; Organic Compounds:804.1 ; Organic Polymers:815.1.1

Web of Science

Southern Univ Sci & Technol, Dept Biomed Engn, 1088 Xueyuan Ave, Shenzhen 518055, Guangdong, Peoples R China

Ye, Huijun,Xian, Yiwen,Li, Shurong,et al. In situ forming injectable gamma-poly(glutamic acid)/PEG adhesive hydrogels for hemorrhage control[J]. Biomaterials Science,2022,10:4218-4227.

Ye, Huijun,Xian, Yiwen,Li, Shurong,Zhang, Chong,&Wu, Decheng.(2022).In situ forming injectable gamma-poly(glutamic acid)/PEG adhesive hydrogels for hemorrhage control.Biomaterials Science,10,4218-4227.

Ye, Huijun,et al."In situ forming injectable gamma-poly(glutamic acid)/PEG adhesive hydrogels for hemorrhage control".Biomaterials Science 10(2022):4218-4227.