Cryo-EM studies of the apo states of human IGF1R

Zhang，Xi1,2,3; Wu，Cang4; Wei，Tianzi4,5; Lu，Yi6; Liu，Chuang7; Zhang，Jian6

2022-08-27

10.1016/j.bbrc.2022.05.063

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Impact Factor & Quartile

0006-291X

1090-2104

IGF1R plays an important role in regulating cellular metabolism and growth. As a single transmembrane protein, its structure is flexible. Although previous studies revealed some structures of IGF1R, the cryo-EM apo structures of the receptor have never been reported. Herein, we reported four distinct cryo-EM structures that reveal the apo states of IGF1R. These conformations were classified as “Resting states” and “Active states”, according to the orientation of α-CT helices and structural symmetry. In addition, a “Ligand-pocket” was formed in the active conformations, which presented a new view of conformational changes of apo-IGF1R. These results suggest a new dynamic change model to show the details of why and how ligands can bind to IGF1R.

Apo-IGF1R Conformation change Cryo-EM

SCI

English

Corresponding

National Natural Science Foundation of China[31900868];National Natural Science Foundation of China[81972766];National Natural Science Foundation of China[JCYJ20170412152943794];National Natural Science Foundation of China[JCYJ20170412154619484];National Natural Science Foundation of China[JCYJ20200109141638004];

Biochemistry & Molecular Biology ; Biophysics

Biochemistry & Molecular Biology ; Biophysics

WOS:000878139200002

ACADEMIC PRESS INC ELSEVIER SCIENCE

BIOLOGY & BIOCHEMISTRY

2-s2.0-85132739372

Scopus

1.Department of Gastroenterology,The First Affiliated Hospital of Shenzhen University,Shenzhen People's Second Hospital,Shenzhen,Guangdong,518000,China
2.Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong,518055,China
3.Cryo-EM Centre,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
4.Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
5.School of Biomedical Sciences,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong SAR, Hong Kong SAR,999077,Hong Kong
6.School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
7.Centre for PanorOmic Sciences,LKS Faculty of Medicine,The University of Hong Kong,Hong Kong SAR, Hong Kong SAR,999077,Hong Kong

Zhang，Xi,Wu，Cang,Wei，Tianzi,et al. Cryo-EM studies of the apo states of human IGF1R[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2022,618:148-152.

Zhang，Xi,Wu，Cang,Wei，Tianzi,Lu，Yi,Liu，Chuang,&Zhang，Jian.(2022).Cryo-EM studies of the apo states of human IGF1R.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,618,148-152.

Zhang，Xi,et al."Cryo-EM studies of the apo states of human IGF1R".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 618(2022):148-152.