中文版 | English
Title

Improved profiling of low molecular weight serum proteome for gastric carcinoma by data-independent acquisition

Author
Corresponding AuthorQin, Yin; Lin, Lin; Liu, Wenlan
Publication Years
2022-06-01
DOI
Source Title
ISSN
1618-2642
EISSN
1618-2650
Volume414Pages:6403-6417
Abstract

Low molecular weight proteins (LMWPs) in the bloodstream participate in various biological processes and are closely associated with disease status, whereas identification of serous LMWPs remains a great technical challenge due to the wide dynamic range of protein components. In this study, we constructed an integrated LMWP library by combining the LMWPs obtained by three enrichment methods (50% ACN, 20% ACN + 20 mM ABC, and 30 kDa) and their fractions identified by the data-dependent acquisition method. With this newly constructed library, we comprehensively profiled LMWPs in serum using data-independent acquisition and reliably achieved quantitative results for 75% serous LMWPs. When applying this strategy to quantify LMWPs in human serum samples, we could identify 405 proteins on average per sample, of which 136 proteins were with a MW less than 30 kDa and 293 proteins were with a MW less than 65 kDa. Of note, pre- and post-operative gastric carcinoma (GC) patients showed differentially expressed serous LWMPs, which was also different from the pattern of LWMP expression in healthy controls. In conclusion, our results showed that LMWPs could efficiently distinguish GC patients from healthy controls as well as between pre- and post-operative statuses, and more importantly, our newly developed LMWP profiling platform could be used to discover candidate LMWP biomarkers for disease diagnosis and status monitoring.

Keywords
URL[Source Record]
Indexed By
SCI ; EI
Language
English
SUSTech Authorship
Corresponding
Funding Project
National Natural Science Foundation of Guangdong Province[
WOS Research Area
Biochemistry & Molecular Biology ; Chemistry
WOS Subject
Biochemical Research Methods ; Chemistry, Analytical
WOS Accession No
WOS:000819264200002
Publisher
EI Accession Number
20222712315341
EI Keywords
Diagnosis ; Molecular Biology ; Molecular Weight
ESI Classification Code
Medicine And Pharmacology:461.6 ; Biology:461.9 ; Organic Compounds:804.1 ; Atomic And Molecular Physics:931.3
ESI Research Field
CHEMISTRY
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/353404
DepartmentPublic Testing and Analysis Center
Affiliation
1.Shenzhen Univ, Cent Lab, Shenzhen Second Peoples Hosp, Affiliated Hosp 1, Shenzhen 518035, Peoples R China
2.Shenzhen Univ, Dept Gastrointestinal Surg, Shenzhen Second Peoples Hosp, Affiliated Hosp 1, Shenzhen 518035, Peoples R China
3.Southern Univ Sci & Technol, Sustech Core Res Facil, Shenzhen 518055, Peoples R China
Corresponding Author AffilicationPublic Testing and Analysis Center
Recommended Citation
GB/T 7714
Li, Weifeng,Li, Mengna,Zhang, Xiaoli,et al. Improved profiling of low molecular weight serum proteome for gastric carcinoma by data-independent acquisition[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2022,414:6403-6417.
APA
Li, Weifeng.,Li, Mengna.,Zhang, Xiaoli.,Yue, Siqin.,Xu, Yun.,...&Liu, Wenlan.(2022).Improved profiling of low molecular weight serum proteome for gastric carcinoma by data-independent acquisition.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,414,6403-6417.
MLA
Li, Weifeng,et al."Improved profiling of low molecular weight serum proteome for gastric carcinoma by data-independent acquisition".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 414(2022):6403-6417.
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