Title | Improved profiling of low molecular weight serum proteome for gastric carcinoma by data-independent acquisition |
Author | |
Corresponding Author | Qin, Yin; Lin, Lin; Liu, Wenlan |
Publication Years | 2022-06-01
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DOI | |
Source Title | |
ISSN | 1618-2642
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EISSN | 1618-2650
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Volume | 414Pages:6403-6417 |
Abstract | Low molecular weight proteins (LMWPs) in the bloodstream participate in various biological processes and are closely associated with disease status, whereas identification of serous LMWPs remains a great technical challenge due to the wide dynamic range of protein components. In this study, we constructed an integrated LMWP library by combining the LMWPs obtained by three enrichment methods (50% ACN, 20% ACN + 20 mM ABC, and 30 kDa) and their fractions identified by the data-dependent acquisition method. With this newly constructed library, we comprehensively profiled LMWPs in serum using data-independent acquisition and reliably achieved quantitative results for 75% serous LMWPs. When applying this strategy to quantify LMWPs in human serum samples, we could identify 405 proteins on average per sample, of which 136 proteins were with a MW less than 30 kDa and 293 proteins were with a MW less than 65 kDa. Of note, pre- and post-operative gastric carcinoma (GC) patients showed differentially expressed serous LWMPs, which was also different from the pattern of LWMP expression in healthy controls. In conclusion, our results showed that LMWPs could efficiently distinguish GC patients from healthy controls as well as between pre- and post-operative statuses, and more importantly, our newly developed LMWP profiling platform could be used to discover candidate LMWP biomarkers for disease diagnosis and status monitoring. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
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SUSTech Authorship | Corresponding
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Funding Project | National Natural Science Foundation of Guangdong Province[
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WOS Research Area | Biochemistry & Molecular Biology
; Chemistry
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WOS Subject | Biochemical Research Methods
; Chemistry, Analytical
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WOS Accession No | WOS:000819264200002
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Publisher | |
EI Accession Number | 20222712315341
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EI Keywords | Diagnosis
; Molecular Biology
; Molecular Weight
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ESI Classification Code | Medicine And Pharmacology:461.6
; Biology:461.9
; Organic Compounds:804.1
; Atomic And Molecular Physics:931.3
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ESI Research Field | CHEMISTRY
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Data Source | Web of Science
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Citation statistics |
Cited Times [WOS]:0
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Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/353404 |
Department | Public Testing and Analysis Center |
Affiliation | 1.Shenzhen Univ, Cent Lab, Shenzhen Second Peoples Hosp, Affiliated Hosp 1, Shenzhen 518035, Peoples R China 2.Shenzhen Univ, Dept Gastrointestinal Surg, Shenzhen Second Peoples Hosp, Affiliated Hosp 1, Shenzhen 518035, Peoples R China 3.Southern Univ Sci & Technol, Sustech Core Res Facil, Shenzhen 518055, Peoples R China |
Corresponding Author Affilication | Public Testing and Analysis Center |
Recommended Citation GB/T 7714 |
Li, Weifeng,Li, Mengna,Zhang, Xiaoli,et al. Improved profiling of low molecular weight serum proteome for gastric carcinoma by data-independent acquisition[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2022,414:6403-6417.
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APA |
Li, Weifeng.,Li, Mengna.,Zhang, Xiaoli.,Yue, Siqin.,Xu, Yun.,...&Liu, Wenlan.(2022).Improved profiling of low molecular weight serum proteome for gastric carcinoma by data-independent acquisition.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,414,6403-6417.
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MLA |
Li, Weifeng,et al."Improved profiling of low molecular weight serum proteome for gastric carcinoma by data-independent acquisition".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 414(2022):6403-6417.
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