Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis

Zhao，Liang1; Wang，Hong1; Fu，Jun2; Wu，Xia1; Liang，Xiao ye3; Liu，Xue yu1; Wu，Xue1,4; Cao，Liang liang1; Xu，Zhen yu5; Dong，Ming1

2022-10-15

10.1016/j.bios.2022.114487

BIOSENSORS & BIOELECTRONICS   Impact Factor & Quartile

0956-5663

1873-4235

Non-invasive methods of detecting cancer by circulating exosomes are challenged by inefficient purification and identification. This study hereby proposed an automated centrifugal microfluidic disc system combined with functionalized membranes (Exo-CMDS) to isolate and enrich exosomes, which will then be processed by a novel aptamer fluorescence system (Exo-AFS) in order to detect the exosome surface proteins in an effective manner. Exo-CMDS features in highly qualified yields with optimal exosomal concentration of 5.1 x 109 particles/mL from trace amount of blood samples (<300 mu L) in only 8 min, which truly accomplishes the exosome isolation and purification in one-step methods. Meanwhile, the limit of detection (LOD) of PD-L1 in Exo-AFS reaches as low as 1.58 x 105 particles/mL. In the trial of clinical samples, the diagnostic accuracy of lung cancer achieves 91% (95% CI: 79%-96%) in contrast to the exosome ELISA (area under the curve: 0.9378 versus 0.8733; 30 patients). Exo-CMDS and Exo-AFS display the precedence in the aspects of inexpensiveness, celerity, purity, sensitivity and specificity when compared with the traditional techniques. Such assays potentially grant a practicable way of detecting inchoate cancers and guiding immunotherapy in clinic.

Circulating biomarkers Exosome Microfluidic Aptamer Lung cancer detection

SCI ; EI

English

Others

Start-up Foundation of Guangzhou Laboratory[YW-JCZD0101]

Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics

Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology

WOS:000826361500005

ELSEVIER ADVANCED TECHNOLOGY

20222712313542

Biological organs ; Diagnosis ; Diseases ; Noninvasive medical procedures ; Proteins ; Purification

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Microfluidics:632.5.1 ; Organic Compounds:804.1

CHEMISTRY

2-s2.0-85133192366

Web of Science

1.Guangzhou Laboratory,Guangzhou,Guangdong Province,510005,China
2.Department of Thoracic Surgery,The Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei Province,430014,China
3.Department of Oncology,Maoming People's Hospital,Maoming,Guangdong Province,525000,China
4.Department of Biomedical Engineering,Southern University of Science and Technology (SUSTech),Shenzhen,518055,China
5.Zhao Rui Medical Biotechnology (GuangZhou) Co. Ltd,510030,China

Zhao，Liang,Wang，Hong,Fu，Jun,et al. Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis[J]. BIOSENSORS & BIOELECTRONICS,2022,214.

Zhao，Liang.,Wang，Hong.,Fu，Jun.,Wu，Xia.,Liang，Xiao ye.,...&Dong，Ming.(2022).Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis.BIOSENSORS & BIOELECTRONICS,214.

Zhao，Liang,et al."Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis".BIOSENSORS & BIOELECTRONICS 214(2022).