Characterization of the Therapeutic Properties and Flavor Profile of Coffee via Monoculture Fermentation with Endophytic Microbial Isolates

Liu，Feng1; Song，Zixian1,3; Zhang，Tongzhou1; Tong，Xinzhi1; Chen，Matthew Y.4; Gao，Dong5; Chen，Jun1,2; Ho，Chun Loong1,2

2022

10.1021/acsfoodscitech.2c00108

ACS Food Science and Technology   Impact Factor & Quartile

2692-1944

Coffee bean fermentation improves coffee flavor and intensity variation using naturally endophytes on cherry surfaces to facilitate fermentation. In this study, we isolated four strains from the parchment and endosperm of green coffee beans: three Bacilli (PMT-1, Bacillus cereus; PMT-2, Priestia aryabhattai; EDS-1, Bacillus subtilis) and one yeast (EDS-2, Pichia guilliermondii). We used two commercially available yeasts (Saccharomyces cerevisiae BY4741, Saccharomyces boulardii lyo) as control. The fermented coffee beans' anti-inflammatory chlorogenic acid was increased by 38.5 and 51.5% when fermented with PMT-1 and EDS-1, respectively, compared to unfermented beans. Yeast fermentation resulted in lower caffeine levels (38%) compared to unfermented beans. PMT-1 and EDS-1 fermented coffee obtained the highest sensory analysis score in sweetness, after-taste, and clean-cup. Additionally, we investigated the PMT-1 and PMT-2 whole-genome sequence to identify these health-benefiting genes. This study provides a feasible approach to increasing the health-promoting constituents in coffee via monoculture fermentation.

Bacillus caffeine chlorogenic acid coffee controlled fermentation yeasts

English

First ; Corresponding

2-s2.0-85133459828

Scopus

1.Department of Biomedical Engineering,Southern University of Science and Technology (SUSTech),Shenzhen,518055,China
2.Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology (SIAT),Chinese Academy of Sciences,Shenzhen,518055,China
3.Department of Orthopaedics & Traumatology,Stem Cells and Regenerative Medicine Laboratory,Li Ka Shing Institute of Health Sciences,Prince of Wales Hospital,The Chinese University of Hong Kong,Shatin,999077,Hong Kong
4.Buyan Coffee Culture Co. Ltd.,Shenzhen,518055,China
5.Shenzhen Puxiang Investment Co. Ltd.,Shenzhen,518055,China

Liu，Feng,Song，Zixian,Zhang，Tongzhou,et al. Characterization of the Therapeutic Properties and Flavor Profile of Coffee via Monoculture Fermentation with Endophytic Microbial Isolates[J]. ACS Food Science and Technology,2022.

Liu，Feng.,Song，Zixian.,Zhang，Tongzhou.,Tong，Xinzhi.,Chen，Matthew Y..,...&Ho，Chun Loong.(2022).Characterization of the Therapeutic Properties and Flavor Profile of Coffee via Monoculture Fermentation with Endophytic Microbial Isolates.ACS Food Science and Technology.

Liu，Feng,et al."Characterization of the Therapeutic Properties and Flavor Profile of Coffee via Monoculture Fermentation with Endophytic Microbial Isolates".ACS Food Science and Technology (2022).