Cryo-EM structures of recombinant human sodium-potassium pump determined in three different states

Guo，Yingying1,2,3; Zhang，Yuanyuan1,2; Yan，Renhong1,2,3; Huang，Bangdong1,2; Ye，Fangfei1,2; Wu，Liushu1,2; Chi，Ximin1,2; shi，Yi1,2; Zhou，Qiang1,2

2022-07-08

10.1038/s41467-022-31602-y

NATURE COMMUNICATIONS   Impact Factor & Quartile

2041-1723

["Sodium-Potassium Pump (Na+/K+-ATPase, NKA) generates an electrochemical gradient of sodium and potassium ions across the plasma membrane by hydrolyzing ATP. Here, the authors report structures of human NKA providing insight into the cytoplasmic Na+ entrance and the cytoplasmic gate closure coupled to ATP hydrolysis.","Sodium-Potassium Pump (Na+/K+-ATPase, NKA) is an ion pump that generates an electrochemical gradient of sodium and potassium ions across the plasma membrane by hydrolyzing ATP. During each Post-Albers cycle, NKA exchanges three cytoplasmic sodium ions for two extracellular potassium ions through alternating changes between the E1 and E2 states. Hitherto, several steps remained unknown during the complete working cycle of NKA. Here, we report cryo-electron microscopy (cryo-EM) structures of recombinant human NKA (hNKA) in three distinct states at 2.7-3.2 angstrom resolution, representing the E1 center dot 3Na and E1 center dot 3Na center dot ATP states with cytosolic gates open and the basic E2 center dot[2K] state, respectively. This work provides the insights into the cytoplasmic Na+ entrance pathway and the mechanism of cytoplasmic gate closure coupled with ATP hydrolysis, filling crucial gaps in the structural elucidation of the Post-Albers cycle of NKA."]

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National Key R&D Program of China[2020YFA0509300] ; National Natural Science Foundation of China["31800139","31971123"]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000825867200009

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1.Westlake Laboratory of Life Sciences and Biomedicine,Key Laboratory of Structural Biology of Zhejiang Province,School of Life Sciences,Westlake University,Hangzhou,18 Shilongshan Road, Zhejiang Province,310024,China
2.Institute of Biology,Westlake Institute for Advanced Study,Hangzhou,18 Shilongshan Road, Zhejiang Province,310024,China
3.Department of Biochemistry,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,China

Guo，Yingying,Zhang，Yuanyuan,Yan，Renhong,et al. Cryo-EM structures of recombinant human sodium-potassium pump determined in three different states[J]. NATURE COMMUNICATIONS,2022,13(1).

Guo，Yingying.,Zhang，Yuanyuan.,Yan，Renhong.,Huang，Bangdong.,Ye，Fangfei.,...&Zhou，Qiang.(2022).Cryo-EM structures of recombinant human sodium-potassium pump determined in three different states.NATURE COMMUNICATIONS,13(1).

Guo，Yingying,et al."Cryo-EM structures of recombinant human sodium-potassium pump determined in three different states".NATURE COMMUNICATIONS 13.1(2022).