tert-Butylhydroquinone-induced formation of high-molecular-weight p62: A novel mechanism in the activation of Nrf2-Keap1
The respiratory system is always exposed to air and is most vulnerable to attack by environmental free radicals. The nuclear factor E2-related factor 2-Kelch-like ECH-associated protein 1-antioxidant response element (Nrf2-Keap1-ARE) pathway and p62 are both involved in the oxidative stress response. However, the interplay between these two systems remains largely unknown. This study shows that treatment of L2 cells with tert-Butylhydroquinone (tBHQ) generates a high-molecular-weight (HMW) form of p62, leading to activation of the Nrf2-Keap1-ARE pathway. The levels of HMW-p62 increased as the tBHQ concentration increased, with concomitant decreases seen in the classical form of p62. Moreover, small interfering RNA targeting p62 increases Keap1 protein levels and inactivates the Nrf2-Keap1-ARE pathway. These results demonstrate that the Nrf2-Keap1 pathway is partially regulated by p62. tBHQ-induced HMW-p62 production may be a novel mechanism in the activation of the Nrf2-Keap1-ARE pathway.
Basic Research (Free Exploration) Project of Shenzhen Science and Technology Plan[JCYJ20190806151610947] ; Sanming project cultivation funds of Shenzhen People's Hospital[SYJY201903]
|WOS Research Area|
|WOS Accession No|
|ESI Research Field|
MOLECULAR BIOLOGY & GENETICS
Web of Science
Cited Times [WOS]:1
|Document Type||Journal Article|
|Department||Shenzhen People's Hospital|
1.Department of Oncology,Shenzhen People's Hospital,The Second Clinical Medical College,Jinan University,Guangdong,China
2.Department of Oncology,The First Affiliated Hospital,Southern University of Science and Technology,Shenzen,China
|First Author Affilication||Shenzhen People's Hospital|
|Corresponding Author Affilication||Shenzhen People's Hospital|
Chen，Yunfang,Cao，Hua,He，Wan,et al. tert-Butylhydroquinone-induced formation of high-molecular-weight p62: A novel mechanism in the activation of Nrf2-Keap1[J]. CELL BIOLOGY INTERNATIONAL,2022.
Chen，Yunfang,Cao，Hua,He，Wan,Zhang，Xi,&Xu，Ruilian.(2022).tert-Butylhydroquinone-induced formation of high-molecular-weight p62: A novel mechanism in the activation of Nrf2-Keap1.CELL BIOLOGY INTERNATIONAL.
Chen，Yunfang,et al."tert-Butylhydroquinone-induced formation of high-molecular-weight p62: A novel mechanism in the activation of Nrf2-Keap1".CELL BIOLOGY INTERNATIONAL (2022).
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