Fluorescence Anisotropy as a Self-Referencing Readout for Ion-Selective Sensing and Imaging Using Homo-FRET between Chromoionophores

Huang, Wenyu; Guo, Chao; Zhai, Jingying; Xie, Xiaojiang

2022-07-12

10.1021/acs.analchem.2c01532

ANALYTICAL CHEMISTRY   Impact Factor & Quartile

0003-2700

1520-6882

ABSTRACT: Fluorescence anisotropy has been widely used in developing biosensors and immunoassays, by virtue of the selfreference and environment-sensitive properties. However, fluorescence anisotropic chemical sensors on inorganic ions are limited by the total anisotropy change. To this end, we demonstrate here fluorescence anisotropic ion-selective optodes based on the homoFRET (Fo''rster resonance energy transfer) of the crowded chromoionophores. The conventional fluorescence on-off mode is transformed into the anisotropic mode. Variation of the target ion concentration changes the inter-chromoionophore distance in the organic sensing phase, leading to different extents of homoFRET and steady-state anisotropy. A theoretical model is developed by coupling homo-FRET and anisotropy. Anisotropic detections of pH, K+, and Na+ are demonstrated as examples based on the different ionophores for H+, K+, and Na+, respectively. Further, fluorescence imaging of the nano-optodes, plasticized poly(vinyl chloride) sensing films, and live cells are demonstrated using a homemade fluorescence anisotropic imaging platform. The results form the basis of an ion-selective analytical method operating in the fluorescence anisotropic mode, which could potentially be applied to other fluorescence on-off probes based on homo-FRET.

SCI ; EI

English

NI Journal Papers

First ; Corresponding

National Natural Science Foundation of China[21874063] ; Shenzhen Municipal Science and Technology Innovation Council[JCYJ20180504165819965]

Chemistry

Chemistry, Analytical

WOS:000823477000001

AMER CHEMICAL SOC

20223012417937

Anisotropy ; Chlorine compounds ; Energy transfer ; Fluorescence imaging ; Fluorophores ; Ions ; Polyvinyl chlorides

Light/Optics:741.1 ; Imaging Techniques:746 ; Physical Chemistry:801.4 ; Inorganic Compounds:804.2 ; Organic Polymers:815.1.1 ; Classical Physics; Quantum Theory; Relativity:931 ; Physical Properties of Gases, Liquids and Solids:931.2

CHEMISTRY

Web of Science

Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China

Huang, Wenyu,Guo, Chao,Zhai, Jingying,et al. Fluorescence Anisotropy as a Self-Referencing Readout for Ion-Selective Sensing and Imaging Using Homo-FRET between Chromoionophores[J]. ANALYTICAL CHEMISTRY,2022,94(27):9793-9800.

Huang, Wenyu,Guo, Chao,Zhai, Jingying,&Xie, Xiaojiang.(2022).Fluorescence Anisotropy as a Self-Referencing Readout for Ion-Selective Sensing and Imaging Using Homo-FRET between Chromoionophores.ANALYTICAL CHEMISTRY,94(27),9793-9800.

Huang, Wenyu,et al."Fluorescence Anisotropy as a Self-Referencing Readout for Ion-Selective Sensing and Imaging Using Homo-FRET between Chromoionophores".ANALYTICAL CHEMISTRY 94.27(2022):9793-9800.