A novel protein-based supramolecular recognition approach for ratiometric fluorescence detection of fipronil
Fipronil, a wildly used pesticide, exhibits high toxicity to pollinators, aquatic organisms, farmland birds, as well as mammals that have GABA receptors, including humans. Thus, developing an effective method for fipronil determination is of great importance. Although the fluorescence assays have the features of easy-to-use, rapid response, high sensitivity and visualized readout, such assay for fipronil was rather difficult to design because fipronil was both chemically and photophysically stable. Herein, we utilized a chalcone dye bound into albumin inner-cavity to construct a novel supramolecular recognition system, for the first time, successfully realizing ratiometric fluorescence detection of fipronil. The sensing mechanism has been carefully verified by the molecular docking, fluorescent titration, DLS tests and fluorescence lifetime. This reported probe could detect fipronil with a rapid response (< 3 min), high sensitivity (LOD similar to 0.01 ppm) and selectivity, good anti-interference property, and significantly, ratiometric color change from green to yellow, which could be discriminated by naked eyes. Moreover, we demonstrated the application of this method in the ratiometric tracking of fipronil in plants (Arabidopsis thaliana). We anticipate this albumin-based supramolecular recognition system would inspire more probe design for environmental monitoring.
National Natural Science Foundation of China["31701826","22077084","32102243"] ; Basic and Applied Basic Research Foundation of Guangdong["2019B030316021","2020A1515011564"] ; Science and Technology Program of Guangzhou ; Postdoctoral Science Foundation of China[2021M691084] ; Shenzhen Basic Research Foundation[JCYJ20210324094010028]
|WOS Research Area|
Chemistry ; Electrochemistry ; Instruments & Instrumentation
Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
|WOS Accession No|
|EI Accession Number|
Aquatic organisms ; Fluorescence imaging ; Probes ; Supramolecular chemistry
|ESI Classification Code|
Marine Science and Oceanography:471 ; Imaging Techniques:746 ; Physical Chemistry:801.4
|ESI Research Field|
Web of Science
Cited Times [WOS]:5
|Document Type||Journal Article|
|Department||Department of Chemistry|
1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education,South China Agricultural University,Guangzhou,510642,China
2.College of Material Science and Engineering,Shenzhen University,Shenzhen,518060,China
3.Department of Chemistry,Southern University of Science and Technology,Shenzhen,518055,China
4.School of Physics,The University of Sydney,2006,Australia
Qin，Tianyi,Zhao，Xiongfei,Jia，Tianhao,et al. A novel protein-based supramolecular recognition approach for ratiometric fluorescence detection of fipronil[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,369.
Qin，Tianyi.,Zhao，Xiongfei.,Jia，Tianhao.,Du，Xinfeng.,Lv，Taoyuze.,...&Zhao，Chen.(2022).A novel protein-based supramolecular recognition approach for ratiometric fluorescence detection of fipronil.SENSORS AND ACTUATORS B-CHEMICAL,369.
Qin，Tianyi,et al."A novel protein-based supramolecular recognition approach for ratiometric fluorescence detection of fipronil".SENSORS AND ACTUATORS B-CHEMICAL 369(2022).
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