Efficient management of the nitritation-anammox microbiome through intermittent aeration: absence of the NOB guild and expansion and diversity of the NOx reducing guild suggests a highly reticulated nitrogen cycle

Palomo, Alejandro1,3; Azevedo, Daniela1; Touceda-Suarez, Maria1,5; Domingo-Felez, Carlos1; Mutlu, A. Gizem1,4; Dechesne, Arnaud1; Wang, Yulin2; Zhang, Tong2; Smets, Barth F.1

2022-07-22

10.1186/s40793-022-00432-2

ENVIRONMENTAL MICROBIOME   Impact Factor & Quartile

2524-6372

Obtaining efficient autotrophic ammonia removal (aka partial nitritation-anammox, or PNA) requires a balanced microbiome with abundant aerobic and anaerobic ammonia oxidizing bacteria and scarce nitrite oxidizing bacteria. Here, we analyzed the microbiome of an efficient PNA process that was obtained by sequential feeding and periodic aeration. The genomes of the dominant community members were inferred from metagenomes obtained over a 6 month period. Three Brocadia spp. genomes and three Nitrosomonas spp. genomes dominated the autotrophic community; no NOB genomes were retrieved. Two of the Brocadia spp. genomes lacked the genomic potential for nitrite reduction. A diverse set of heterotrophic genomes was retrieved, each with genomic potential for only a fraction of the denitrification pathway. A mutual dependency in amino acid and vitamin synthesis was noted between autotrophic and heterotrophic community members. Our analysis suggests a highly-reticulated nitrogen cycle in the examined PNA microbiome with nitric oxide exchange between the heterotrophs and the anammox guild.

Nitritation Anammox Ammonia Nitrite Brocadia Nitrosomonas Nitric oxide

SCI

English

Others

Research Project LaGas[12-132633] ; Research Centre EcoDesign[09-067230] ; EU-FP7 People: Marie-Curie Actions[REA 607492] ; Villlum Fonden[Expa-N 13391]

Genetics & Heredity ; Microbiology

Genetics & Heredity ; Microbiology

WOS:000829034300001

BMC

2-s2.0-85134620570

Web of Science

1.Tech Univ Denmark, Dept Environm Engn, Microbial Ecol & Technol Lab, Lyngby, Denmark
2.Univ Hong Kong, Dept Civil Engn, Environm Microbiome Engn & Biotechnol Lab, Hong Kong, Peoples R China
3.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Shenzhen, Guangdong, Peoples R China
4.Hydrotech Veolia Water Technol, Vellinge, Sweden
5.Univ Arizona, Dept Environm Sci, Tucson, AZ USA

Palomo, Alejandro,Azevedo, Daniela,Touceda-Suarez, Maria,et al. Efficient management of the nitritation-anammox microbiome through intermittent aeration: absence of the NOB guild and expansion and diversity of the NOx reducing guild suggests a highly reticulated nitrogen cycle[J]. ENVIRONMENTAL MICROBIOME,2022,17(1).

Palomo, Alejandro.,Azevedo, Daniela.,Touceda-Suarez, Maria.,Domingo-Felez, Carlos.,Mutlu, A. Gizem.,...&Smets, Barth F..(2022).Efficient management of the nitritation-anammox microbiome through intermittent aeration: absence of the NOB guild and expansion and diversity of the NOx reducing guild suggests a highly reticulated nitrogen cycle.ENVIRONMENTAL MICROBIOME,17(1).

Palomo, Alejandro,et al."Efficient management of the nitritation-anammox microbiome through intermittent aeration: absence of the NOB guild and expansion and diversity of the NOx reducing guild suggests a highly reticulated nitrogen cycle".ENVIRONMENTAL MICROBIOME 17.1(2022).