中文版 | English
Title

Comprehensive Analysis of the Transcriptome-wide m6A Methylome in Lung Adenocarcinoma by MeRIP Sequencing

Author
Corresponding AuthorWang,Yukun
Publication Years
2022-07-11
DOI
Source Title
ISSN
2234-943X
Volume12
Abstract

N6-methyladenosine (m6A) is the most abundant internal modification on eukaryotic mRNAs. There is increasing evidence that m6A plays a key role in tumor progression, so it is important to analyze m6A modifications within the transcriptome-wide in lung adenocarcinoma (LUAD). Three pairs of LUAD samples and tumor-adjacent normal tissues were obtained from the South University of Science and Technology Hospital. And then methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were used to identify differential m6A modifications between tumor and tumor-adjacent normal tissues. We identified 4041 aberrant m6A peaks, of which 1192 m6A peaks were upregulated and 2849 m6A peaks downregulated. It was found that genes with the dysregulated m6A peaks were enriched in the pathways in cancer, Rap1 signaling pathway, and insulin resistance. Additionally, 612 genes with abnormal regulation of m6A peaks and RNA expression were identified by combining MeRIP-seq and RNA-seq data. Through KEGG analysis, the 612 genes were enriched in cancer-related signaling pathways, such as the cGMP-PKG signaling pathway, and the Rap1 signaling pathway. What's more, GSEA enrichment analysis showed these genes were enriched in cell cycle phase transition, cell division, cellular response to DNA damage stimulus, and chromosome organization. To further explore the relationship between differential m6A modified genes and clinical parameters of LUAD patients, we searched The Cancer Genome Atlas (TCGA) and identified 2 genes (FCRL5 and GPRIN1) that were associated with the prognosis and diagnosis of LUAD patients. Furthermore, we found a positive correlation between GPRIN1 and m6A reader YTHDF1 in the GEPIA2 database. It was verified that YTHDF1 binds to GPRIN1 mRNA and regulates its expression. Our study results suggest that m6A modification plays important role in the progression and prognosis of LUAD and maybe a potential new therapeutic target for LUAD patients in the future.

Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
First ; Corresponding
WOS Research Area
Oncology
WOS Subject
Oncology
WOS Accession No
WOS:000832695500001
Publisher
Scopus EID
2-s2.0-85134657163
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:2
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/359556
DepartmentDepartment of Pharmacology
南方科技大学医学院
南方科技大学医院
Affiliation
1.Department of Pharmacology,School of Medicine,Southern University of Science and Technology,Shenzhen,China
2.Medical Research Center,Southern University of Science and Technology Hospital,Shenzhen,China
3.Nutrition Department,Southern University of Science and Technology Hospital,Shenzhen,China
4.Department of Clinical Laboratory,Southern University of Science and Technology Hospital,Shenzhen,China
5.Department of Pharmacy,Southern University of Science and Technology Hospital,Shenzhen,China
First Author AffilicationDepartment of Pharmacology;  School of Medicine
Corresponding Author AffilicationDepartment of Pharmacology;  School of Medicine;  Southern University of Science and Technology Hospital
First Author's First AffilicationDepartment of Pharmacology;  School of Medicine
Recommended Citation
GB/T 7714
Mao,Wenli,Yu,Qingzhen,Wang,Kefeng,et al. Comprehensive Analysis of the Transcriptome-wide m6A Methylome in Lung Adenocarcinoma by MeRIP Sequencing[J]. Frontiers in Oncology,2022,12.
APA
Mao,Wenli.,Yu,Qingzhen.,Wang,Kefeng.,Ma,Qiang.,Zheng,Yuxin.,...&Wang,Yukun.(2022).Comprehensive Analysis of the Transcriptome-wide m6A Methylome in Lung Adenocarcinoma by MeRIP Sequencing.Frontiers in Oncology,12.
MLA
Mao,Wenli,et al."Comprehensive Analysis of the Transcriptome-wide m6A Methylome in Lung Adenocarcinoma by MeRIP Sequencing".Frontiers in Oncology 12(2022).
Files in This Item:
File Name/Size DocType Version Access License
Comprehensive Analys(6234KB) Open Access--View
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Export to Excel
Export to Csv
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Mao,Wenli]'s Articles
[Yu,Qingzhen]'s Articles
[Wang,Kefeng]'s Articles
Baidu Scholar
Similar articles in Baidu Scholar
[Mao,Wenli]'s Articles
[Yu,Qingzhen]'s Articles
[Wang,Kefeng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Mao,Wenli]'s Articles
[Yu,Qingzhen]'s Articles
[Wang,Kefeng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Comprehensive Analysis of the Transcriptome-wide m6A Methylome in Lung Adenocarcinoma by MeRIP Sequencing.pdf
Format: Adobe PDF
File name: Comprehensive Analysis of the Transcriptome-wide m6A Methylome in Lung Adenocarcinoma by MeRIP Sequencing.pdf
Format: Adobe PDF
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.