The Role of AIF-1 in the Aldosterone-Induced Vascular Calcification Related to Chronic Kidney Disease: Evidence From Mice Model and Cell Co-Culture Model

Chang, Xueying1; Hao, Jianbing2; Wang, Xingzhi1; Liu, Jingwei1; Ni, Jie1; Hao, Lirong1

2022-07-20

10.3389/fendo.2022.917356

Frontiers in Endocrinology   Impact Factor & Quartile

1664-2392

Increasing evidence suggests that aldosterone (Aldo) plays an essential role in vascular calcification which is a serious threat to cardiovascular disease (CVD) developed from chronic kidney disease (CKD). However, the exact pathogenesis of vascular calcification is still unclear. First, we established CKD-associated vascular calcification mice model and knockout mice model to investigate the causal relationship between allograft inflammatory factor 1 (AIF-1) and vascular calcification. Then, endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) co-culture experiments were performed to further explore the mechanisms of calcification. The results of the Aldo intervention mice model and transgenic mice model showed that Aldo could cause calcification by increasing the AIF-1 level. The results of in vitro co-culture model of ECs and VSMCs showed that AIF-1 silence in ECs may alleviate Aldo-induced calcification of VSMCs. In conclusion, our study indicated that Aldo may induce vascular calcification related to chronic renal failure via the AIF-1 pathway which may provide a potential therapeutic target.

aldosterone allograft inflammatory factor-1 vascular calcification chronic kidney disease cell co-culture

SCI

English

Others

National Natural Science Foundation of China[81870503] ; Heilongjiang Natural Fund joint guiding project[LH2019H025]

Endocrinology & Metabolism

Endocrinology & Metabolism

WOS:000836172400001

FRONTIERS MEDIA SA

Web of Science

1.Harbin Med Univ, Dept Nephropathy & Hemodialysis, Affiliated Hosp 1, Harbin, Peoples R China
2.Southern Univ Sci & Technol Hosp, Dept Nephropathy, Shenzhen, Peoples R China

Chang, Xueying,Hao, Jianbing,Wang, Xingzhi,et al. The Role of AIF-1 in the Aldosterone-Induced Vascular Calcification Related to Chronic Kidney Disease: Evidence From Mice Model and Cell Co-Culture Model[J]. Frontiers in Endocrinology,2022,13.

Chang, Xueying,Hao, Jianbing,Wang, Xingzhi,Liu, Jingwei,Ni, Jie,&Hao, Lirong.(2022).The Role of AIF-1 in the Aldosterone-Induced Vascular Calcification Related to Chronic Kidney Disease: Evidence From Mice Model and Cell Co-Culture Model.Frontiers in Endocrinology,13.

Chang, Xueying,et al."The Role of AIF-1 in the Aldosterone-Induced Vascular Calcification Related to Chronic Kidney Disease: Evidence From Mice Model and Cell Co-Culture Model".Frontiers in Endocrinology 13(2022).