Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis

Gao, Ruru1; Luo, Qiong1; Li, Yang2,3; Song, Liming4; Cai, Junnan (Stephen)5; Xiong, Ying1; Yan, Fei6; Liu, Jianhua1

2022-07-01

10.3390/pharmaceutics14071382

PHARMACEUTICS   Impact Factor & Quartile

1999-4923

The epithelial-mesenchymal transition (EMT), a process in which epithelial cells undergo a series of biochemical changes to acquire a mesenchymal phenotype, has been linked to tumor metastasis. Here, we present a novel strategy for knocking out the EMT-related Cdh2 gene, which encodes N-cadherin through CRISPR/Cas9-mediated gene editing by an ultrasound combined with biosynthetic nanobubbles (Gas Vesicles, GVs). Polyethyleneimine were employed as a gene delivery vector to deliver sgRNA into 4T1 cells that stably express the Cas9 protein, resulting in the stable Cdh2 gene- knockout cell lines. The Western blotting assay confirmed the absence of an N-cadherin protein in these Cdh2 gene-knockout 4T1 cell lines. Significantly reduced tumor cell migration was observed in the Cdh2 gene-knockout 4T1 cells in comparison with the wild-type cells. Our study demonstrated that an ultrasound combined with GVs could effectively mediate CRISPR/Cas9 gene editing of a Cdh2 gene to inhibit tumor invasion and metastasis.

ultrasound CRISPR Cas9 gene editing epithelial-mesenchymal transition N-cadherin

SCI

English

Others

National Key Research and Development Program of China[2020YFA0908800] ; National Natural Science Foundation of China["81871376","82071935","81901771"] ; Science, Technology and Innovation Commission of Shenzhen Municipality["JCYJ20190812171820731","JCYJ20190807144209381"]

Pharmacology & Pharmacy

Pharmacology & Pharmacy

WOS:000833387700001

MDPI

Web of Science

1.South China Univ Technol, Affiliated Hosp 2, Sch Med, Dept Med Ultrasound, Guangzhou 510180, Peoples R China
2.Jinan Univ, Shenzhen Peoples Hosp, Dept Gastrointestinal Surg, Clin Med Coll 2, Shenzhen 518020, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Dept Gastrointestinal Surg, Affiliated Hosp 1, Shenzhen 518020, Peoples R China
4.Southern Med Univ, Zhujiang Hosp, Dept Orthoped, Guangzhou 510282, Peoples R China
5.SXUltrason Shenzhen Ltd, Shenzhen 518000, Peoples R China
6.Chinese Acad Sci, Ctr Cell & Gene Circuit Design, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol,CAS Key Lab Quantitat E, Shenzhen 518055, Peoples R China

Gao, Ruru,Luo, Qiong,Li, Yang,et al. Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis[J]. PHARMACEUTICS,2022,14(7).

Gao, Ruru.,Luo, Qiong.,Li, Yang.,Song, Liming.,Cai, Junnan .,...&Liu, Jianhua.(2022).Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis.PHARMACEUTICS,14(7).

Gao, Ruru,et al."Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis".PHARMACEUTICS 14.7(2022).