In vivo processing of digital information molecularly with targeted specificity and robust reliability

Liu，Yangyi1; Ren，Yubin1; Li，Jingjing2; Wang，Fan2; Wang，Fei3; Ma，Chao1; Chen，Dong4; Jiang，Xingyu5; Fan，Chunhai3; Zhang，Hongjie1,2; Liu，Kai1,2

2022-08-05

10.1126/sciadv.abo7415

Science Advances   Impact Factor & Quartile

2375-2548

2375-2548

DNA has attracted increasing interest as an appealing medium for information storage. However, target-specific rewriting of the digital data stored in intracellular DNA remains a grand challenge because the highly repetitive nature and uneven guanine-cytosine content render the encoded DNA sequences poorly compatible with endogenous ones. In this study, a dual-plasmid system based on gene editing tools was introduced into Escherichia coli to process information accurately. Digital data containing large repeat units in binary codes, such as text, codebook, or image, were involved in the realization of target-specific rewriting in vivo, yielding up to 94% rewriting reliability. An optical reporter was introduced as an advanced tool for presenting data processing at the molecular level. Rewritten information was stored stably and amplified over hundreds of generations. Our work demonstrates a digital-to-biological information processing approach for highly efficient data storage, amplification, and rewriting, thus robustly promoting the application of DNA-based information technology.

SCI ; EI

English

NI Journal Papers

Others

National Key Research and Development Program of China["2018YFA0902600","2021YFF1200300","2020YFA0712102","2021YFB3502300"] ; National Natural Science Foundation of China["22125701","21877104","21834007","22107097","21878258","22020102003"] ; Tsinghua University Spring Breeze Fund[2021Z99CFZ005] ; Zhejiang Provincial Natural Science Foundation of China[Y20B060027] ; Beijing Nova Program[Z211100002121132] ; Beijing Natural Science Foundation[22220101]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000836990600035

AMER ASSOC ADVANCEMENT SCIENCE

20223212549391

Data handling ; Digital storage ; DNA sequences ; Escherichia coli

Biological Materials and Tissue Engineering:461.2 ; Data Storage, Equipment and Techniques:722.1 ; Data Processing and Image Processing:723.2

2-s2.0-85135087368

Scopus

1.Department of Chemistry,Tsinghua University,Beijing,China
2.State Key Laboratory of Rare Earth Resource Utilization,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,China
3.Frontiers Science Center for Transformative Molecules,School of Chemistry and Chemical Engineering,Institute of Molecular Medicine,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai,China
4.College of Energy Engineering and State Key Laboratory of Fluid Power and Mechatronic Systems,Zhejiang University,Hangzhou,China
5.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,No. 1088 Xueyuan Road, Nanshan District, Guangdong,China

Liu，Yangyi,Ren，Yubin,Li，Jingjing,et al. In vivo processing of digital information molecularly with targeted specificity and robust reliability[J]. Science Advances,2022,8(31).

Liu，Yangyi.,Ren，Yubin.,Li，Jingjing.,Wang，Fan.,Wang，Fei.,...&Liu，Kai.(2022).In vivo processing of digital information molecularly with targeted specificity and robust reliability.Science Advances,8(31).

Liu，Yangyi,et al."In vivo processing of digital information molecularly with targeted specificity and robust reliability".Science Advances 8.31(2022).