中文版 | English
Title

Real-time 3D imaging of ocean algae with crosstalk suppressed single-shot digital holographic microscopy

Author
Corresponding AuthorHe,Hao
Publication Years
2022-08-01
DOI
Source Title
ISSN
2156-7085
EISSN
2156-7085
Volume13Issue:8Pages:4455-4467
Abstract
Digital holographic microscopy (DHM) has the potential to reconstruct the 3D shape of volumetric samples from a single-shot hologram in a label-free and noninvasive manner. However, the holographic reconstruction is significantly compromised by the out-of-focus image resulting from the crosstalk between refocused planes, leading to the low fidelity of the results. In this paper, we propose a crosstalk suppression algorithm-assisted 3D imaging method combined with a home built DHM system to achieve accurate 3D imaging of ocean algae using only a single hologram. As a key step in the algorithm, a hybrid edge detection strategy using gradient-based and deep learning-based methods is proposed to offer accurate boundary information for the downstream processing. With this information, the crosstalk of each refocused plane can be estimated with adjacent refocused planes. Empowered by this method, we demonstrated successful 3D imaging of six kinds of ocean algae that agree well with the ground truth; we further demonstrated that this method could achieve real-time 3D imaging of the quick swimming ocean algae in the water environment. To our knowledge, this is the first time single-shot DHM is reported in 3D imaging of ocean algae, paving the way for on-site monitoring of the ocean algae.
URL[Source Record]
Indexed By
SCI ; EI
Language
English
SUSTech Authorship
Corresponding
WOS Research Area
Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS Subject
Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS Accession No
WOS:000841999800008
Publisher
EI Accession Number
20223212542348
EI Keywords
Crosstalk ; Holograms ; Image reconstruction ; Imaging systems ; Microscopic examination ; Oceanography
ESI Classification Code
Oceanography, General:471.1 ; Holography:743 ; Imaging Techniques:746
Scopus EID
2-s2.0-85135416442
Data Source
Scopus
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/375634
DepartmentDepartment of Biomedical Engineering
Affiliation
1.School of Advanced Manufacturing,Nanchang University,Nanchang,Jiangxi,330031,China
2.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
Corresponding Author AffilicationDepartment of Biomedical Engineering
Recommended Citation
GB/T 7714
Tang,Ming,He,Hao,Yu,Longkun. Real-time 3D imaging of ocean algae with crosstalk suppressed single-shot digital holographic microscopy[J]. Biomedical Optics Express,2022,13(8):4455-4467.
APA
Tang,Ming,He,Hao,&Yu,Longkun.(2022).Real-time 3D imaging of ocean algae with crosstalk suppressed single-shot digital holographic microscopy.Biomedical Optics Express,13(8),4455-4467.
MLA
Tang,Ming,et al."Real-time 3D imaging of ocean algae with crosstalk suppressed single-shot digital holographic microscopy".Biomedical Optics Express 13.8(2022):4455-4467.
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