中文版 | English
Title

A Modified SMART-Seq Method for Single-Cell Transcriptomic Analysis of Embryoid Body Differentiation

Author
Corresponding AuthorChen,Xi
Publication Years
2022
ISBN
978-1-0716-2436-4
Source Title
Publication Place
UK
Publisher
Volume
2520
Pages
233-259
Abstract

Embryoid bodies (EBs) are aggregate of cells that contain three embryonic germ layers. They can be formed by direct differentiation from pluripotent embryonic stem cells (ESCs), which serves as a useful model for understanding early embryo development. Due to the mixture of different cell types, it is necessary to investigate EBs at the single-cell level. Here, we describe a robust and straightforward method for single-cell gene expression profiling during mouse EB differentiation from mouse ESCs (mESCs). The protocol is modified from a widely used method in the SMART-seq family, which only requires standard molecular biology techniques and lab equipment. It allows for accurate 3′ counting of transcript at the single-cell level, which helps reveal cellular identities during EB formation. Combined with perturbation experiments, the method provides an opportunity for mechanistic studies of embryo development at the single-cell level.

Keywords
ISSN
1064-3745
EISSN
1940-6029
Scopus EID
2-s2.0-85135282572
DOI
URL[Source Record]
Language
English
SUSTech Authorship
First ; Corresponding
Data Source
Scopus
Citation statistics
Cited Times [WOS]:0
Document TypeBook chapter
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/375666
DepartmentSchool of Life Sciences
Affiliation
1.Shenzhen Key Laboratory of Gene Regulation and Systems Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,China
2.Department of Physiology,School of Basic Medical Sciences,Binzhou Medical University,Yantai,China
3.Cam-Su Genomic Resource Center,Soochow University,Suzhou,China
First Author AffilicationSchool of Life Sciences
Corresponding Author AffilicationSchool of Life Sciences
Recommended Citation
GB/T 7714
Zheng,Jianqun,Ye,Ying,Xu,Qiushi,et al. A Modified SMART-Seq Method for Single-Cell Transcriptomic Analysis of Embryoid Body Differentiation. UK:University of Hertfordshire Hatfield,2022:233-259.
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