Title | A Modified SMART-Seq Method for Single-Cell Transcriptomic Analysis of Embryoid Body Differentiation |
Author | |
Corresponding Author | Chen,Xi |
Publication Years | 2022
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ISBN | 978-1-0716-2436-4
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Source Title | |
Publication Place | UK
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Publisher | |
Volume | 2520
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Pages | 233-259
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Abstract | Embryoid bodies (EBs) are aggregate of cells that contain three embryonic germ layers. They can be formed by direct differentiation from pluripotent embryonic stem cells (ESCs), which serves as a useful model for understanding early embryo development. Due to the mixture of different cell types, it is necessary to investigate EBs at the single-cell level. Here, we describe a robust and straightforward method for single-cell gene expression profiling during mouse EB differentiation from mouse ESCs (mESCs). The protocol is modified from a widely used method in the SMART-seq family, which only requires standard molecular biology techniques and lab equipment. It allows for accurate 3′ counting of transcript at the single-cell level, which helps reveal cellular identities during EB formation. Combined with perturbation experiments, the method provides an opportunity for mechanistic studies of embryo development at the single-cell level. |
Keywords | |
ISSN | 1064-3745
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EISSN | 1940-6029
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Scopus EID | 2-s2.0-85135282572
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DOI | |
URL | [Source Record] |
Language | English
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SUSTech Authorship | First
; Corresponding
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Data Source | Scopus
|
Citation statistics |
Cited Times [WOS]:0
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Document Type | Book chapter |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/375666 |
Department | School of Life Sciences |
Affiliation | 1.Shenzhen Key Laboratory of Gene Regulation and Systems Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,China 2.Department of Physiology,School of Basic Medical Sciences,Binzhou Medical University,Yantai,China 3.Cam-Su Genomic Resource Center,Soochow University,Suzhou,China |
First Author Affilication | School of Life Sciences |
Corresponding Author Affilication | School of Life Sciences |
Recommended Citation GB/T 7714 |
Zheng,Jianqun,Ye,Ying,Xu,Qiushi,et al. A Modified SMART-Seq Method for Single-Cell Transcriptomic Analysis of Embryoid Body Differentiation. UK:University of Hertfordshire Hatfield,2022:233-259.
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