| Title | miR-133a-5p Inhibits Glioma Cell Proliferation by Regulating IGFBP3 |
| Author | |
| Corresponding Author | Lv, Wen |
| Publication Years | 2022-08-02
|
| DOI | |
| Source Title | |
| ISSN | 1687-8450
|
| EISSN | 1687-8469
|
| Volume | 2022 |
| Abstract | Objective. This research aims to investigate the expression of miR-133a-5p in glioma tissues and its impact on glioma cell proliferation. Methods. Fluorescence-quantitative PCR was used to detect the expression of miR-133a-5p in 25 cases of glioma and adjuncent tissues. CCK-8 and colony formation analyses were used to evaluate the impact of transfection with miR-133a-5p inhibitors or mimics on glioma cell growth and colony formation. The IGFBP3 (insulin-like growth factor-binding protein-3) and miR-133a-5p binding sites were predicted using Starbase, and the miR-133a-5p binding capacity with 3'UTR of IGFBP3 gene was determined using a luciferase gene reporter system. Following transfection with miR-133a-5p mimics or inhibitors, the IGFBP3 protein expression in glioma cells was determined by western blotting. The colony formation assay was applied to evaluate the influence of IGFBP3 overexpression on the miR-133a-5p in glioma cell proliferation. For assessment of the IGFBP3 expression in glioma tissues and prognosis, TCGA database was employed. Results. The expression of miR-133a-5p was considerably reduced in glioma tissue compared to adjuncent control tissue. In addition, miR-133a-5p expression decreased with increasing glioma malignancy. Glioma cell growth and colony formation were reduced after miR-133a-5p mimics were transfected, while transfection of miR-133a-5p inhibitors had a reverse impact. The expression of IGFBP3 was affected by miR-133a-5p by binding to its 3'UTR region. Additional study demonstrated that the overall survival (OS) of subjects with increased IGFBP3 expression was considerably lower compared to patients with decreased IGFBP3 expression. The IGFBP3 overexpression effectively counteracts the glioma cell proliferation-inhibiting impact of miR-133a-5p. Conclusion. miR-133a-5p acts as a glioma tumor suppressor gene. It reduces glioma cell proliferation by modulating IGFBP3 and could be a target for glioma therapy. |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | Corresponding
|
| Funding Project | [JCYJ20210324114008023]
|
| WOS Research Area | Oncology
|
| WOS Subject | Oncology
|
| WOS Accession No | WOS:000840759000001
|
| Publisher | |
| Data Source | Web of Science
|
| Citation statistics |
Cited Times [WOS]:0
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/382305 |
| Department | Shenzhen People's Hospital |
| Affiliation | 1.Southern Med Univ, Dept Neurosurg, Shenzhen Hosp, Shenzhen, Guangdong, Peoples R China 2.Shenzhen Yantian Dists Peoples Hosp, Dept Neurosurg, Shenzhen, Guangdong, Peoples R China 3.Shenzhen Peoples Hosp, Dept Neurosurg, Shenzhen, Guangdong, Peoples R China 4.Guangdong Engn Technol Res Ctr Nervous Anat & Rela, Zhongshan, Peoples R China 5.Univ South China, Dept Neurosurg, Changsha Cent Hosp, Changsha, Hunan, Peoples R China 6.Southern Univ Sci & Technol, Affiliated Hosp 1, Dept Neurosurg, Shenzhen, Guangdong, Peoples R China |
| Corresponding Author Affilication | Shenzhen People's Hospital |
| Recommended Citation GB/T 7714 |
Yang, Xinzhi,Chen, Dong,Hu, Jiliang,et al. miR-133a-5p Inhibits Glioma Cell Proliferation by Regulating IGFBP3[J]. Journal of Oncology,2022,2022.
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| APA |
Yang, Xinzhi,Chen, Dong,Hu, Jiliang,Zhao, Qingsuo,Fu, Xing,&Lv, Wen.(2022).miR-133a-5p Inhibits Glioma Cell Proliferation by Regulating IGFBP3.Journal of Oncology,2022.
|
| MLA |
Yang, Xinzhi,et al."miR-133a-5p Inhibits Glioma Cell Proliferation by Regulating IGFBP3".Journal of Oncology 2022(2022).
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