In vivo visualization and analysis of ciliary motion in allergic rhinitis models induced by ovalbumin

Liu，Chen1,2,3,4; Pang，Chuan5; Chen，Dai Shi6; Wang，Jin1,2,3,4; Yi，Wen Qi1,2,3,4; Yu，Ning1,2,3,4; Chen，Lei1,2,3,4

2022

10.1177/15353702221088781

EXPERIMENTAL BIOLOGY AND MEDICINE   Impact Factor & Quartile

1535-3702

1535-3699

Due to the lack of an assessment approach, the image of in vivo nasal ciliary motion of allergic rhinitis (AR) has never been captured and analyzed to date. Here, we have used an optimized approach to analyze the nasal ciliary function in vivo in AR rats. The digital microscopy system, a method for direct observation of ciliary motion in a living AR rat model, was applied to visualize and measure ciliary motion in vivo, including ciliary beat frequency (CBF) and ciliary beat distance (CBD). The AR rat model was established by ovalbumin sensitization. Comparisons of nasal ciliary motion in vivo between the experimental group (ovalbumin sensitization, allergen, or histamine) and the control group were analyzed. In the living rat model of allergic rhinitis, CBF and CBD decreased to 57.8 and 73.1% of the control group, respectively, but were restored after administration of chlorpheniramine maleate. Ovalbumin (OVA) significantly inhibited the ciliary motion of normal mucosa in vivo. However, responding to the OVA challenge, the ciliary motion of OVA-sensitized mucosa would not decrease further and stay at a stable level. Histamine stimulated in vivo ciliary motion quickly within 30 min, but afterward, the ciliary motion gradually decreased below the baseline. These results have clarified that in vivo ciliary motion was impaired by nasal mucosal sensitization, and this impairment was most likely related to allergen challenge and histamine. In addition, the short-term stimulation and long-term inhibition effects of histamine on in vivo ciliary motion were first reported in this study.

In vivo allergic rhinitis ciliary motility histamine microscopy nasal mucosa

SCI

English

Others

WOS:000795803100001

CLINICAL MEDICINE

2-s2.0-85132662249

Scopus

1.Senior Department of Otolaryngology-Head Neck Surgery,The Sixth Medical Center of PLA General Hospital,Beijing,100048,China
2.National Clinical Research Center for Otolaryngologic Diseases,Beijing,100048,China
3.State Key Lab of Hearing Science,Ministry of Education,Beijing,100048,China
4.Beijing Key Lab of Hearing Impairment for Prevention and Treatment,Beijing,100048,China
5.Department of General Surgery,First Medical Center of Chinese,PLA General Hospital,Beijing,100853,China
6.Department of Otorhinolaryngology,Shenzhen People’s Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,518020,China

Liu，Chen,Pang，Chuan,Chen，Dai Shi,et al. In vivo visualization and analysis of ciliary motion in allergic rhinitis models induced by ovalbumin[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2022.

Liu，Chen.,Pang，Chuan.,Chen，Dai Shi.,Wang，Jin.,Yi，Wen Qi.,...&Chen，Lei.(2022).In vivo visualization and analysis of ciliary motion in allergic rhinitis models induced by ovalbumin.EXPERIMENTAL BIOLOGY AND MEDICINE.

Liu，Chen,et al."In vivo visualization and analysis of ciliary motion in allergic rhinitis models induced by ovalbumin".EXPERIMENTAL BIOLOGY AND MEDICINE (2022).