The carboxy terminal transmembrane domain of SPL7 mediates interaction with RAN1 at the endoplasmic reticulum to regulate ethylene signalling in Arabidopsis

Yang, Yanzhi1,2; Hao, Chen1,2; Du, Jianmei3; Xu, Lei3; Guo, Zhonglong1,2; Li, Dong4; Cai, Huaqing4; Guo, Hongwei5; Li, Lei1,2,3

2022-08-01

10.1111/nph.18376

NEW PHYTOLOGIST   Impact Factor & Quartile

0028-646X

1469-8137

In Arabidopsis, copper (Cu) transport to the ethylene receptor ETR1 mediated using RAN1, a Cu transporter located at the endoplasmic reticulum (ER), and Cu homeostasis mediated using SPL7, the key Cu-responsive transcription factor, are two deeply conserved vital processes. However, whether and how the two processes interact to regulate plant development remain elusive. We found that its C-terminal transmembrane domain (TMD) anchors SPL7 to the ER, resulting in dual compartmentalisation of the transcription factor. Immunoprecipitation coupled mass spectrometry, yeast-two-hybrid assay, luciferase complementation imaging and subcellular co-localisation analyses indicate that SPL7 interacts with RAN1 at the ER via the TMD. Genetic analysis revealed that the ethylene-induced triple response was significantly compromised in the spl7 mutant, a phenotype rescuable by RAN1 overexpression but not by SPL7 without the TMD. The genetic interaction was corroborated by molecular analysis showing that SPL7 modulates RAN1 abundance in a TMD-dependent manner. Moreover, SPL7 is feedback regulated by ethylene signalling via EIN3, which binds the SPL7 promoter and represses its transcription. These results demonstrate that ER-anchored SPL7 constitutes a cellular mechanism to regulate RAN1 in ethylene signalling and lay the foundation for investigating how Cu homeostasis conditions ethylene sensitivity in the developmental context.

Arabidopsis copper homeostasis ethylene RAN1 SPL7 triple response

SCI

English

Others

National Key Research and Development Programme of China["2018YFE0204700","2017YFA0503800"] ; National Natural Science Foundation of China[31621001]

Plant Sciences

Plant Sciences

WOS:000840894400001

WILEY

PLANT & ANIMAL SCIENCE

Web of Science

1.Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China
2.Peking Univ, Sch Adv Agr Sci, Beijing 100871, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
4.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China
5.Southern Univ Sci & Technol, Inst Plant & Food Sci, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China

Yang, Yanzhi,Hao, Chen,Du, Jianmei,et al. The carboxy terminal transmembrane domain of SPL7 mediates interaction with RAN1 at the endoplasmic reticulum to regulate ethylene signalling in Arabidopsis[J]. NEW PHYTOLOGIST,2022.

Yang, Yanzhi.,Hao, Chen.,Du, Jianmei.,Xu, Lei.,Guo, Zhonglong.,...&Li, Lei.(2022).The carboxy terminal transmembrane domain of SPL7 mediates interaction with RAN1 at the endoplasmic reticulum to regulate ethylene signalling in Arabidopsis.NEW PHYTOLOGIST.

Yang, Yanzhi,et al."The carboxy terminal transmembrane domain of SPL7 mediates interaction with RAN1 at the endoplasmic reticulum to regulate ethylene signalling in Arabidopsis".NEW PHYTOLOGIST (2022).