Parental transfer of an antibiotic mixture induces cardiotoxicity in early life-stage zebrafish: A cross-generational study

Xuan，Rongrong1; Qiu，Wenhui2,3; Zhou，Yuping1; Magnuson，Jason T.4; Luo，Shusheng3; Greer，Justin B.4; Xu，Bentuo5; Liu，Jingyu3; Xu，Elvis Genbo6; Schlenk，Daniel4; Zheng，Chunmiao3

2022-11-25

10.1016/j.scitotenv.2022.157726

SCIENCE OF THE TOTAL ENVIRONMENT   Impact Factor & Quartile

0048-9697

1879-1026

Antibiotic residues in the aquatic environment have been shown to induce significant adverse effects on the early-life stage development of aquatic organisms, though the underlying molecular mechanisms of these effects have not been well characterized. In this study, we performed global mRNA-miRNA sequencing, canonical pathway analyses, morphological, physiological, immunohistochemical, and behavioral analyses to comprehensively assess the cross-generational cardiotoxicity and mechanisms of antibiotic mixtures in zebrafish. Following parental treatment to 1 and 100 μg/L antibiotic mixtures (15 of the most commonly detected antibiotics) for 150 days, all 15 assessed antibiotics were detected in the F1 eggs, indicating the cross-generational transfer of antibiotics. Global mRNA-miRNA sequencing functional analysis predicted cardiotoxicity in the F1 generation by using the F1 whole fish. Consistent with canonical pathway analyses, significant cardiotoxicity was observed in F1 larvae, as well as the apoptosis of cardiac cells. Furthermore, let-7a-5p regulated the cardiac hypertrophy signaling pathway, suggesting mechanisms of miRNA of let-7 family mediating cross-generational cardiotoxicity of antibiotics in zebrafish. This study lays some groundwork for developing interventions to prevent parental exposure to environmental pollutants such as antibiotics from adversely affecting offspring development.

SCI ; EI

English

Corresponding

Basic and Applied Basic Research Foundation of Guangdong Province[2020A1515010891];Ningbo Municipal Bureau of Science and Technology[2021S101];National Key Research and Development Program of China[2021YFA1202500];National Natural Science Foundation of China[42077223];

Environmental Sciences & Ecology

Environmental Sciences

WOS:000863316400010

ELSEVIER

20223412594402

Aquatic organisms ; Cell death ; Mixtures ; RNA

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biology:461.9 ; Marine Science and Oceanography:471

ENVIRONMENT/ECOLOGY

2-s2.0-85135916638

Scopus

1.The Affiliated Hospital of Medical School,Ningbo University,Ningbo,315020,China
2.School of Public Health and Emergency Management,Southern University of Science and Technology,Shenzhen,518055,China
3.State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control,Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control,School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
4.Department of Environmental Sciences,University of California,Riverside,92521,United States
5.School of Life and Environmental Science,Wenzhou University,Wenzhou,325035,China
6.Department of Biology,University of Southern Denmark,Odense,5230,Denmark

Xuan，Rongrong,Qiu，Wenhui,Zhou，Yuping,et al. Parental transfer of an antibiotic mixture induces cardiotoxicity in early life-stage zebrafish: A cross-generational study[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,849.

Xuan，Rongrong.,Qiu，Wenhui.,Zhou，Yuping.,Magnuson，Jason T..,Luo，Shusheng.,...&Zheng，Chunmiao.(2022).Parental transfer of an antibiotic mixture induces cardiotoxicity in early life-stage zebrafish: A cross-generational study.SCIENCE OF THE TOTAL ENVIRONMENT,849.

Xuan，Rongrong,et al."Parental transfer of an antibiotic mixture induces cardiotoxicity in early life-stage zebrafish: A cross-generational study".SCIENCE OF THE TOTAL ENVIRONMENT 849(2022).