Interactions between Membraneless Condensates and Membranous Organelles at the Presynapse: A Phase Separation View of Synaptic Vesicle Cycle

Wu，Xiandeng1; Qiu，Hua1; Zhang，Mingjie2,3

2023-01-15

10.1016/j.jmb.2022.167629

JOURNAL OF MOLECULAR BIOLOGY   Impact Factor & Quartile

0022-2836

1089-8638

Action potential-induced neurotransmitter release in presynaptic boutons involves coordinated actions of a large list of proteins that are associated directly or indirectly with membrane structures including synap-tic vesicles and plasma membranes. These proteins are often highly abundant in different synaptic bouton sub-compartments, and they rarely act alone. Instead, these proteins interact with each other forming intri-cate and distinct molecular complexes. Many of these complexes form condensed clusters on membrane surfaces. This review summarizes findings in recent years showing that many of presynaptic protein com-plex assemblies are formed via phase separation. These protein condensates extensively interact with lipid membranes via distinct modes, forming various mesoscale structures by different mode of organiza-tions between membraneless condensates and membranous organelles. We discuss that such mesos-cale interactions could have deep implications on mobilization, exocytosis, and retrieval of synaptic vesicles. (c) 2022 Elsevier Ltd. All rights reserved.

SCI

English

Corresponding

Minister of Science and Technology of China[2019YFA0508402] ; National Natural Science Foundation of China[82188101] ; Shenzhen Bay Laboratory[S201101002] ; Research Grant Council of Hong Kong["AoE-M09-12","16104518","16101419"]

Biochemistry & Molecular Biology

Biochemistry & Molecular Biology

WOS:000915722700001

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Division of Life Science,Hong Kong University of Science and Technology,Kowloon,Clear Water Bay,Hong Kong
2.Greater Bay Biomedical Innocenter,Shenzhen Bay Laboratory,Shenzhen,518036,China
3.School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China

Wu，Xiandeng,Qiu，Hua,Zhang，Mingjie. Interactions between Membraneless Condensates and Membranous Organelles at the Presynapse: A Phase Separation View of Synaptic Vesicle Cycle[J]. JOURNAL OF MOLECULAR BIOLOGY,2023,435(1).

Wu，Xiandeng,Qiu，Hua,&Zhang，Mingjie.(2023).Interactions between Membraneless Condensates and Membranous Organelles at the Presynapse: A Phase Separation View of Synaptic Vesicle Cycle.JOURNAL OF MOLECULAR BIOLOGY,435(1).

Wu，Xiandeng,et al."Interactions between Membraneless Condensates and Membranous Organelles at the Presynapse: A Phase Separation View of Synaptic Vesicle Cycle".JOURNAL OF MOLECULAR BIOLOGY 435.1(2023).