Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay

Sun, Wei1,2; Chen, Wei1,3

2018

10.1007/978-1-4939-7540-2_3

Methods in Molecular Biology   Impact Factor & Quartile

1064-3745

The development of genome-wide RNA profiling technologies greatly facilitates the global analysis of gene expression. However, such technologies alone could not distinguish the contribution to cellular RNA abundance by transcription versus decay. To overcome such limitation, metabolic labeling of newly synthesized RNA with 4-thiouridine (4sU) combined with genome-wide RNA profiling was used to in parallel measure RNA transcription and decay. Here, we describe the detailed protocol for using metabolic labeling with 4sU to separate newly synthesized RNA from the preexisting RNA in mammalian cells.

4-Thiouridine(4sU) Metabolic labeling Newly synthesized RNA RNA transcription RNA decay Global analysis

BKCI-S

English

First ; Corresponding

Biochemistry & Molecular Biology ; Genetics & Heredity

Biochemical Research Methods ; Biochemistry & Molecular Biology ; Genetics & Heredity

WOS:000433435600004

HUMANA PRESS INC

Web of Science

1.Southern Univ Sci & Technol, Dept Biol, Shenzhen, Nanshan Distric, Peoples R China
2.Max Delbruck Ctr Mol Med, Lab Funct & Med Genom, Berlin Inst Med Syst Biol, Berlin, Germany
3.Southern Univ Sci & Technol, Medi X Inst, SUSTech Acad Adv Interdisciplinary Studies, Shenzhen, Nanshan Distric, Peoples R China

Sun, Wei,Chen, Wei. Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay[J]. Methods in Molecular Biology,2018,1720:25-34.

Sun, Wei,&Chen, Wei.(2018).Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay.Methods in Molecular Biology,1720,25-34.

Sun, Wei,et al."Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay".Methods in Molecular Biology 1720(2018):25-34.