A CRISPR-based ultrasensitive assay detects attomolar concentrations of SARS-CoV-2 antibodies in clinical samples

Tang, Yanan1; Song, Turun2; Gao, Lu1; Yin, Saifu2; Ma, Ming2; Tan, Yun1; Wu, Lijuan3; Yang, Yang4; Wang, Yanqun5; Lin, Tao2; Li, Feng1,6

2022-08-09

10.1038/s41467-022-32371-4

NATURE COMMUNICATIONS   Impact Factor & Quartile

2041-1723

["CRISPR diagnostics are powerful tools for detecting nucleic acids but are generally not deployable for the detection of clinically important proteins. Here, we report an ultrasensitive CRISPR-based antibody detection (UCAD) assay that translates the detection of anti-SARS-CoV-2 antibodies into CRISPR-based nucleic acid detection in a homogeneous solution and is 10,000 times more sensitive than the classic immunoassays. Clinical validation using serum samples collected from the general population (n = 197), demonstrates that UCAD has 100% sensitivity and 98.5% specificity. With ultrahigh sensitivity, UCAD enables the quantitative analysis of serum anti-SARS-CoV-2 levels in vaccinated kidney transplant recipients who are shown to produce \"undetectable\" anti-SARS-CoV-2 using standard immunoassay. Because of the high sensitivity and simplicity, we anticipate that, upon further clinical validation against large cohorts of clinical samples, UCAD will find wide applications for clinical uses in both centralized laboratories and point-of-care settings.","CRISPR diagnostics are routinely used for the detecting nucleic acids, but rarely for clinically important proteins. Here, by translating a CRISPR-based DNA test into an ultrasensitive assay for antibodies, the authors achieve antibody detection from serum samples at attomolar concentrations."]

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Fundamental Research Funds for the Central Universities[YJ201975] ; National Natural Science Foundation of China["22006104","22074099"] ; Institutional Research Fund from Sichuan University[2021SCUNL105] ; Sichuan Science and Technology Project[2021YJ0322]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000842289800029

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Web of Science

1.Sichuan Univ, Key Lab Green Chem & Technol, Minist Educ, Coll Chem,Analyt & Testing Ctr, Chengdu 610064, Sichuan, Peoples R China
2.Sichuan Univ, West China Hosp, Organ Transplantat Ctr, Urol Dept,Urol Res Inst, Chengdu 610041, Sichuan, Peoples R China
3.Sichuan Univ, West China Hosp, Dept Lab Med, Chengdu 610041, Sichuan, Peoples R China
4.Southern Univ Sci & Technol, Shenzhen Key Lab Pathogen & Immun, Shenzhen Peoples Hosp 3, Hosp 2, Shenzhen, Peoples R China
5.Guangzhou Med Univ, State Key Lab Resp Dis, Natl Clin Res Ctr Resp Dis, Affiliated Hosp 1,Guangzhou Inst Resp Hlth, Guangzhou, Guangdong, Peoples R China
6.Brock Univ, Ctr Biotechnol, Dept Chem, St Catharines, ON L2S 3A1, Canada

Tang, Yanan,Song, Turun,Gao, Lu,et al. A CRISPR-based ultrasensitive assay detects attomolar concentrations of SARS-CoV-2 antibodies in clinical samples[J]. NATURE COMMUNICATIONS,2022,13(1).

Tang, Yanan.,Song, Turun.,Gao, Lu.,Yin, Saifu.,Ma, Ming.,...&Li, Feng.(2022).A CRISPR-based ultrasensitive assay detects attomolar concentrations of SARS-CoV-2 antibodies in clinical samples.NATURE COMMUNICATIONS,13(1).

Tang, Yanan,et al."A CRISPR-based ultrasensitive assay detects attomolar concentrations of SARS-CoV-2 antibodies in clinical samples".NATURE COMMUNICATIONS 13.1(2022).