Integrated single-cell analyses decode the developmental landscape of the human fetal spine

Yu, Haiyan1,2; Tang, Donge1; Wu, Hongwei1; Li, Chunhong1; Lu, Yongping1,3; He, Fang4; Zhang, Xiaogang4; Yang, Yane5; Shi, Wei6; Hu, Wenlong1; Zeng, Zhipeng1; Dai, Weier7; Ou, Minglin8; Dai, Yong1

2022-07-15

10.1016/j.isci.2022.104679

ISCIENCE   Impact Factor & Quartile

2589-0042

The spine has essential roles in supporting body weight, and passaging the neural elements between the body and the brain. In this study, we used integrated sin-gle-cell RNA sequencing and single-cell transposase-accessible chromatin sequencing analyses to reveal the cellular heterogeneity, lineage, and transcrip-tional regulatory network of the developing human spine. We found that EPYC + HAPLN1+ fibroblasts with stem cell characteristics could differentiate into chondrocytes by highly expressing the chondrogenic markers SOX9 and MATN4. Neurons could originate from neuroendocrine cells, and MEIS2 may be an essential transcription factor that promotes spinal neural progenitor cells to selectively differentiate into neurons during early gestation. Furthermore, the interaction of NRP2_SEMA3C and CD74_APP between macrophages and neu-rons may be essential for spinal cord development. Our integrated map provides a blueprint for understanding human spine development in the early and midges-tational stages at single-cell resolution and offers a tool for investigating related diseases.

SCI

English

First ; Corresponding

Science and Technology Planning Project of Guangdong Province, China[2021A1515111071] ; Guangdong Provincial Clinical Research Center for Birth Defects[2017B 020209001]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000838442800009

CELL PRESS

Web of Science

1.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2,Affiliated Hosp 1,Clin Med Res Ct, Shenzhen 518020, Guangdong, Peoples R China
2.Southern Med Univ, Shenzhen Pingshan Dist Peoples Hosp, Dept Pharm, Pingshan Gen Hosp, Shenzhen 518118, Guangdong, Peoples R China
3.Jinan Univ, Affiliated Hosp 1, Inst Nephrol & Blood Purificat, Guangzhou 510632, Peoples R China
4.Singleron Biotechnol, Yaogu Ave 11, Nanjing, Jiangsu, Peoples R China
5.Shenzhen East Women & Children Hosp, Shenzhen 518000, Guangdong, Peoples R China
6.Southern Univ Sci & Technol, Jinan Univ, Clin Med Coll 2, Dept Obstet & Gynecol,Affiliated Hosp 1,Shenzhen, Shenzhen 518020, Guangdong, Peoples R China
7.Univ Texas Austin, Coll Nat Sci, Austin, TX 78721 USA
8.Guilin Med Univ, Cent Lab, Affiliated Hosp 2, 212 Renmin Rd, Guilin 541000, Peoples R China

Yu, Haiyan,Tang, Donge,Wu, Hongwei,et al. Integrated single-cell analyses decode the developmental landscape of the human fetal spine[J]. ISCIENCE,2022,25(7).

Yu, Haiyan.,Tang, Donge.,Wu, Hongwei.,Li, Chunhong.,Lu, Yongping.,...&Dai, Yong.(2022).Integrated single-cell analyses decode the developmental landscape of the human fetal spine.ISCIENCE,25(7).

Yu, Haiyan,et al."Integrated single-cell analyses decode the developmental landscape of the human fetal spine".ISCIENCE 25.7(2022).