Covalent incorporation of metalloporphyrin in luminescent polymer dot transducer for continuous glucose monitoring

Liu，Siyang1,2; Fang，Xiaofeng2; Mi，Feixue2; Sun，Kai2; Wu，Changfeng2

2022-11-01

10.1016/j.jlumin.2022.119202

JOURNAL OF LUMINESCENCE   Impact Factor & Quartile

0022-2313

1872-7883

Luminescent dye-doped polymer dots (Pdots) have been demonstrated for various biosensing applications. However, the dye doping strategy may suffer from dye aggregation and dye leaching issues which limit the long-term sensor performance. Here, we show that covalent incorporation of metalloporphyrin in polyfluorene backbone for continuous glucose monitoring. The resulting polymer was used to develop luminescent Pdots transducer that overcome the dye-aggregation problem. We characterized the spectroscopic properties of the metalloporphyrin covalently-linked Pdots, which showed reduced batch-to-batch variation, enhanced intra-particle energy transfer, and improved luminescence stability as compared to the metalloporphyrin-doped Pdots. After bioconjugation with glucose oxidase (GOx), the oxygen-sensitive phosphorescence is highly sensitive to glucose variations. The metalloporphyrin-linked Pdots transducer showed good performance in long-term continuous glucose monitoring in mouse models. This work demonstrates that covalent incorporation of porphyrin dyes in Pdots transducer is a reliable strategy to improve their stability and reproducibility for long-term continuous glucose monitoring in vivo.

Continuous glucose monitoring Dye-linked polymer dots Glucose transducer

SCI ; EI

English

Corresponding

National Key Research and Development Program of China[2018YFB0407200];State Key Laboratory of Integrated Pest Management[IOSKL2020KF13];Shenzhen Science and Technology Innovation Program[JCYJ20210324115807021];Shenzhen Science and Technology Innovation Program[KQTD20170810111314625];Shenzhen Bay Laboratory[SZBL2021080601002];

Optics

Optics

WOS:000863176000001

ELSEVIER

20223512627619

Agglomeration ; Energy transfer ; Glucose ; Glucose oxidase ; Glucose sensors ; Luminescence ; Metals ; Porphyrins

Biology:461.9 ; Biomedical Equipment, General:462.1 ; Light/Optics:741.1 ; Chemical Operations:802.3 ; Organic Compounds:804.1

PHYSICS

2-s2.0-85136286324

Scopus

1.Harbin Institute of Technology,Harbin,150001,China
2.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China

Liu，Siyang,Fang，Xiaofeng,Mi，Feixue,et al. Covalent incorporation of metalloporphyrin in luminescent polymer dot transducer for continuous glucose monitoring[J]. JOURNAL OF LUMINESCENCE,2022,251.

Liu，Siyang,Fang，Xiaofeng,Mi，Feixue,Sun，Kai,&Wu，Changfeng.(2022).Covalent incorporation of metalloporphyrin in luminescent polymer dot transducer for continuous glucose monitoring.JOURNAL OF LUMINESCENCE,251.

Liu，Siyang,et al."Covalent incorporation of metalloporphyrin in luminescent polymer dot transducer for continuous glucose monitoring".JOURNAL OF LUMINESCENCE 251(2022).