Extracellular pH sensing by plant cell-surface peptide-receptor complexes

Liu，Li1,2; Song，Wen2,3,4; Huang，Shijia4; Jiang，Kai1,5; Moriwaki，Yoshitaka6; Wang，Yichuan1; Men，Yongfan7; Zhang，Dan1; Wen，Xing1; Han，Zhifu4; Chai，Jijie2,3,4; Guo，Hongwei1

2022-09-01

10.1016/j.cell.2022.07.012

CELL   Impact Factor & Quartile

0092-8674

1097-4172

The extracellular pH is a vital regulator of various biological processes in plants. However, how plants perceive extracellular pH remains obscure. Here, we report that plant cell-surface peptide-receptor complexes can function as extracellular pH sensors. We found that pattern-triggered immunity (PTI) dramatically alkalinizes the acidic extracellular pH in root apical meristem (RAM) region, which is essential for root meristem growth factor 1 (RGF1)-mediated RAM growth. The extracellular alkalinization progressively inhibits the acidic-dependent interaction between RGF1 and its receptors (RGFRs) through the pH sensor sulfotyrosine. Conversely, extracellular alkalinization promotes the alkaline-dependent binding of plant elicitor peptides (Peps) to its receptors (PEPRs) through the pH sensor Glu/Asp, thereby promoting immunity. A domain swap between RGFR and PEPR switches the pH dependency of RAM growth. Thus, our results reveal a mechanism of extracellular pH sensing by plant peptide-receptor complexes and provide insights into the extracellular pH-mediated regulation of growth and immunity in the RAM.

alkalinization extracellular pH pattern-triggered immunity Pep1 pH-sensing plant peptide receptor-like kinase RGF1 root meristem sulfotyrosine

SCI

English

NI Journal Papers

First ; Corresponding

National Key Research and Development Program of China[2018YFA0507101];National Key Research and Development Program of China[2019YFA0903904];National Natural Science Foundation of China[31421001];National Natural Science Foundation of China[31971119];National Natural Science Foundation of China[91940303];

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:000858905600002

CELL PRESS

MOLECULAR BIOLOGY & GENETICS

2-s2.0-85136542729

Scopus

1.Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes,Institute of Plant and Food Science,Department of Biology,Southern University of Science and Technology (SUSTech),Shenzhen,Guangdong,518055,China
2.Max-Planck Institute for Plant Breeding Research,Cologne,50829,Germany
3.Institute of Biochemistry,University of Cologne,Cologne,50923,Germany
4.Innovation Center for Structural Biology,School of Life Sciences,Tsinghua University,Beijing,100084,China
5.SUSTech Academy for Advanced and Interdisciplinary Studies,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
6.Department of Biotechnology,Graduate School of Agricultural and Life Sciences,The University of Tokyo,Tokyo,1-1-1 Yayoi, Bunkyo-ku,113-8657,Japan
7.Research Laboratory of Biomedical Optics and Molecular Imaging,Institute of Biomedical and Health Engineering,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong,518055,China

Liu，Li,Song，Wen,Huang，Shijia,et al. Extracellular pH sensing by plant cell-surface peptide-receptor complexes[J]. CELL,2022,185(18):3341-3355.e13.

Liu，Li.,Song，Wen.,Huang，Shijia.,Jiang，Kai.,Moriwaki，Yoshitaka.,...&Guo，Hongwei.(2022).Extracellular pH sensing by plant cell-surface peptide-receptor complexes.CELL,185(18),3341-3355.e13.

Liu，Li,et al."Extracellular pH sensing by plant cell-surface peptide-receptor complexes".CELL 185.18(2022):3341-3355.e13.