Title | Transcriptome Analysis Reveals the Multiple Functions of pBD2 in IPEC-J2 Cells against E. coli |
Author | |
Corresponding Author | Li, Chunli |
Publication Years | 2022-09-01
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DOI | |
Source Title | |
EISSN | 1422-0067
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Volume | 23Issue:17 |
Abstract | Defensins play an important role in fighting bacteria, and are a good candidate for bactericidal agents. However, the function and mechanism of defensins in regulating host responses against bacteria is unclear. In this study, transcriptome analysis was used to study the comprehensive functions of pBD2 in IPEC-J2 cells against E. coli. In total, 230 differentially expressed genes (DEGs) were identified in IPEC-J2 cells between the control and E. coli groups, and were found by KEGG analysis to be involved in many signaling pathways related to immunity. Furthermore, 812 DEGs were observed between E. coli and E. coli +pBD2 groups, involved in the ribosome, oxidative phosphorylation, and certain disease pathways. Among these, 94 overlapping DEGs were in the two DEG groups, and 85 DEGs were reverse expression, which is involved in microRNA in cancer, while PTEN and CDC6 were key genes according to PPI net analysis. The results of qRT-PCR verified those of RNA-seq. The results indicated that pBD2 plays an important role against E. coli by acting on the genes related to immune response, cell cycle, ribosomes, oxidative phosphorylation, etc. The results provide new insights into the potential function and mechanism of pBD2 against E. coli. Meanwhile, this study provides a certain theoretical basis for research and the development of novel peptide drugs. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
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SUSTech Authorship | Others
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Funding Project | Science and Technology Research Project in Henan Province[202102110089]
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WOS Research Area | Biochemistry & Molecular Biology
; Chemistry
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WOS Subject | Biochemistry & Molecular Biology
; Chemistry, Multidisciplinary
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WOS Accession No | WOS:000851110100001
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Publisher | |
ESI Research Field | CHEMISTRY
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Data Source | Web of Science
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Citation statistics |
Cited Times [WOS]:1
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Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/395919 |
Department | School of Medicine |
Affiliation | 1.Henan Agr Univ, Coll Anim Sci & Technol, Zhengzhou 450046, Peoples R China 2.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China |
Recommended Citation GB/T 7714 |
Lian, Shaoqiang,Lin, Xiaqing,Zhan, Fengting,et al. Transcriptome Analysis Reveals the Multiple Functions of pBD2 in IPEC-J2 Cells against E. coli[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(17).
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APA |
Lian, Shaoqiang,Lin, Xiaqing,Zhan, Fengting,Shen, Xiaoyang,Liang, Yu,&Li, Chunli.(2022).Transcriptome Analysis Reveals the Multiple Functions of pBD2 in IPEC-J2 Cells against E. coli.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(17).
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MLA |
Lian, Shaoqiang,et al."Transcriptome Analysis Reveals the Multiple Functions of pBD2 in IPEC-J2 Cells against E. coli".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.17(2022).
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