中文版 | English
Title

Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry

Author
Corresponding AuthorXie, Xiaojiang
Publication Years
2022-09-01
DOI
Source Title
ISSN
1618-2642
EISSN
1618-2650
Abstract
We present here an ionophore-based ion-selective optode (ISO) platform to detect potassium and sodium concentrations in serum through flow cytometry. The ion-selective microsensors were based on polyethylene glycol (PEG)-modified polystyrene (PS) microspheres (PEG-PS). Ratiometric response curves were observed using peak channel fluorescence intensities for K+ (10(-6) M to 0.1 M) and Na+ (10(-4) M to 0.2 M) with sufficient selectivity for clinical diagnosis. Due to the matrix effect, proteins such as albumin and immunoglobulin caused an obvious increase in response for serum sample determination. To solve this problem, 4-arm PEG chains were covalently attached onto the surface of PS microspheres through a two-step reaction, which improved the stability and combated pollution of microspheres. As a preliminary application, potassium and sodium concentrations in human serums were successfully determined by the PEG-PS microsensors through flow cytometry.
Keywords
URL[Source Record]
Indexed By
SCI ; EI
Language
English
SUSTech Authorship
Corresponding
Funding Project
National Natural Science Foundation of China[21874063]
WOS Research Area
Biochemistry & Molecular Biology ; Chemistry
WOS Subject
Biochemical Research Methods ; Chemistry, Analytical
WOS Accession No
WOS:000848008900001
Publisher
EI Accession Number
20223612680615
EI Keywords
Electrolytes ; Ionophores ; Ions ; Microsensors ; Microspheres ; Potassium
ESI Classification Code
Alkali Metals:549.1 ; Electric Batteries and Fuel Cells:702 ; Optical Devices and Systems:741.3 ; Chemical Agents and Basic Industrial Chemicals:803 ; Chemical Products Generally:804 ; Optical Variables Measurements:941.4
ESI Research Field
CHEMISTRY
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/395948
DepartmentDepartment of Chemistry
Affiliation
1.Harbin Inst Technol, Sch Chem & Chem Engn, Harbin 150001, Peoples R China
2.Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China
Corresponding Author AffilicationDepartment of Chemistry
Recommended Citation
GB/T 7714
Du, Xinfeng,Wang, Renjie,Zhai, Jingying,et al. Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2022.
APA
Du, Xinfeng,Wang, Renjie,Zhai, Jingying,&Xie, Xiaojiang.(2022).Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY.
MLA
Du, Xinfeng,et al."Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022).
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