Title | Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry |
Author | |
Corresponding Author | Xie, Xiaojiang |
Publication Years | 2022-09-01
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DOI | |
Source Title | |
ISSN | 1618-2642
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EISSN | 1618-2650
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Abstract | We present here an ionophore-based ion-selective optode (ISO) platform to detect potassium and sodium concentrations in serum through flow cytometry. The ion-selective microsensors were based on polyethylene glycol (PEG)-modified polystyrene (PS) microspheres (PEG-PS). Ratiometric response curves were observed using peak channel fluorescence intensities for K+ (10(-6) M to 0.1 M) and Na+ (10(-4) M to 0.2 M) with sufficient selectivity for clinical diagnosis. Due to the matrix effect, proteins such as albumin and immunoglobulin caused an obvious increase in response for serum sample determination. To solve this problem, 4-arm PEG chains were covalently attached onto the surface of PS microspheres through a two-step reaction, which improved the stability and combated pollution of microspheres. As a preliminary application, potassium and sodium concentrations in human serums were successfully determined by the PEG-PS microsensors through flow cytometry. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
|
SUSTech Authorship | Corresponding
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Funding Project | National Natural Science Foundation of China[21874063]
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WOS Research Area | Biochemistry & Molecular Biology
; Chemistry
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WOS Subject | Biochemical Research Methods
; Chemistry, Analytical
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WOS Accession No | WOS:000848008900001
|
Publisher | |
EI Accession Number | 20223612680615
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EI Keywords | Electrolytes
; Ionophores
; Ions
; Microsensors
; Microspheres
; Potassium
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ESI Classification Code | Alkali Metals:549.1
; Electric Batteries and Fuel Cells:702
; Optical Devices and Systems:741.3
; Chemical Agents and Basic Industrial Chemicals:803
; Chemical Products Generally:804
; Optical Variables Measurements:941.4
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ESI Research Field | CHEMISTRY
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Data Source | Web of Science
|
Citation statistics |
Cited Times [WOS]:0
|
Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/395948 |
Department | Department of Chemistry |
Affiliation | 1.Harbin Inst Technol, Sch Chem & Chem Engn, Harbin 150001, Peoples R China 2.Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China |
Corresponding Author Affilication | Department of Chemistry |
Recommended Citation GB/T 7714 |
Du, Xinfeng,Wang, Renjie,Zhai, Jingying,et al. Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2022.
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APA |
Du, Xinfeng,Wang, Renjie,Zhai, Jingying,&Xie, Xiaojiang.(2022).Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY.
|
MLA |
Du, Xinfeng,et al."Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022).
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