Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry

Du, Xinfeng1; Wang, Renjie2; Zhai, Jingying2; Xie, Xiaojiang1,2

2022-09-01

10.1007/s00216-022-04301-2

ANALYTICAL AND BIOANALYTICAL CHEMISTRY   Impact Factor & Quartile

1618-2642

1618-2650

We present here an ionophore-based ion-selective optode (ISO) platform to detect potassium and sodium concentrations in serum through flow cytometry. The ion-selective microsensors were based on polyethylene glycol (PEG)-modified polystyrene (PS) microspheres (PEG-PS). Ratiometric response curves were observed using peak channel fluorescence intensities for K+ (10(-6) M to 0.1 M) and Na+ (10(-4) M to 0.2 M) with sufficient selectivity for clinical diagnosis. Due to the matrix effect, proteins such as albumin and immunoglobulin caused an obvious increase in response for serum sample determination. To solve this problem, 4-arm PEG chains were covalently attached onto the surface of PS microspheres through a two-step reaction, which improved the stability and combated pollution of microspheres. As a preliminary application, potassium and sodium concentrations in human serums were successfully determined by the PEG-PS microsensors through flow cytometry.

Flow cytometry Blood electrolytes Ionophore Latex beads Surface PEGylation Microsensor

SCI ; EI

English

Corresponding

National Natural Science Foundation of China[21874063]

Biochemistry & Molecular Biology ; Chemistry

Biochemical Research Methods ; Chemistry, Analytical

WOS:000848008900001

SPRINGER HEIDELBERG

20223612680615

Electrolytes ; Ionophores ; Ions ; Microsensors ; Microspheres ; Potassium

Alkali Metals:549.1 ; Electric Batteries and Fuel Cells:702 ; Optical Devices and Systems:741.3 ; Chemical Agents and Basic Industrial Chemicals:803 ; Chemical Products Generally:804 ; Optical Variables Measurements:941.4

CHEMISTRY

Web of Science

1.Harbin Inst Technol, Sch Chem & Chem Engn, Harbin 150001, Peoples R China
2.Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China

Du, Xinfeng,Wang, Renjie,Zhai, Jingying,et al. Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2022.

Du, Xinfeng,Wang, Renjie,Zhai, Jingying,&Xie, Xiaojiang.(2022).Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY.

Du, Xinfeng,et al."Surface PEGylation of ionophore-based microspheres enables determination of serum sodium and potassium ion concentration under flow cytometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022).