Dual Inhibition of HIV-1 and Cathepsin L Proteases by Sarcandra glabra

Pan, Bowen1; Li, Sumei2; Xiao, Junwei1; Yang, Xin1; Xie, Shouxia2; Zhou, Ying1; Yang, Jian3; Wei, Ying1

2022-09-01

10.3390/molecules27175552

MOLECULES   Impact Factor & Quartile

1420-3049

The COVID-19 pandemic continues to impose a huge threat on human health due to rapid viral mutations. Thus, it is imperative to develop more potent antivirals with both prophylactic and treatment functions. In this study, we screened for potential antiviral compounds from Sarcandra glabra (SG) against Cathepsin L and HIV-1 proteases. A FRET assay was applied to investigate the inhibitory effects and UPLC-HRMS was employed to identify and quantify the bioactive components. Furthermore, molecular docking was carried out to get a glimpse of the binding of active compounds to the proteases. Our results showed that the SG extracts (SGW, SG30, SG60, and SG85) inhibited HIV-1 protease with an IC50 of 0.003 similar to 0.07 mg/mL and Cathepsin L protease with an IC50 of 0.11 similar to 0.26 mg/mL. Fourteen compounds were identified along with eight quantified from the SG extracts. Chlorogenic acid, which presented in high content in the extracts (12.7 similar to 15.76 mu g/mg), possessed the most potent inhibitory activity against HIV-1 protease (IC50 = 0.026 mg/mL) and Cathepsin L protease (inhibition: 40.8% at 0.01 mg/mL). Thus, SG extracts and the active ingredients could potentially be used to prevent/treat viral infections, including SARS-CoV-2, due to their dual-inhibition functions against viral proteases.

Sarcandra glabra HIV-1 protease Cathepsin L protease inhibition UPLC-HRMS molecular docking

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English

Others

Guizhou Provincial Science and Technology Projects["(2020)4Y213","ZK [2022] general 480"] ; Innovation Group Project of Guizhou Province[(2021)018] ; Research Center of Molecular Bioactivity from Traditional Chinese Medicine and Ethnomedicine[3411-4110000520364] ; Shenzhen Fund for Guangdong Provincial High-Level Clinical Key Specialties[SZXK059] ; projects of Guizhou Province["gzwkj2022-232","gzwkj2022-467","[2022]253"] ; projects of Guizhou University of Traditional Chinese Medicine["2018YFC170810204","2018YFC170810108"]

Biochemistry & Molecular Biology ; Chemistry

Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary

WOS:000851748300001

MDPI

CHEMISTRY

Web of Science

1.Guizhou Univ Tradit Chinese Med, Coll Pharm, Guiyang 550025, Peoples R China
2.Southern Univ Sci & Technol, Jinan Univ, Affiliated Hosp 1,Dept Pharmacol, Shenzhen Peoples Hosp,Clin Med Coll 2, Shenzhen 518020, Peoples R China
3.Univ Saskatchewan, Coll Pharm & Nutr, 107 Wiggins Rd, Saskatoon, SK S7N 5E5, Canada

Pan, Bowen,Li, Sumei,Xiao, Junwei,et al. Dual Inhibition of HIV-1 and Cathepsin L Proteases by Sarcandra glabra[J]. MOLECULES,2022,27(17).

Pan, Bowen.,Li, Sumei.,Xiao, Junwei.,Yang, Xin.,Xie, Shouxia.,...&Wei, Ying.(2022).Dual Inhibition of HIV-1 and Cathepsin L Proteases by Sarcandra glabra.MOLECULES,27(17).

Pan, Bowen,et al."Dual Inhibition of HIV-1 and Cathepsin L Proteases by Sarcandra glabra".MOLECULES 27.17(2022).