The Hydrophobicity of AIE Dye Facilitates DNA Condensation for Carrier-Free Gene Therapy

Chen, Zhe1,2; Wei, Zixiang1; Xiao, Fan1; Chao, Zhicong1; Lu, Jingxiong1; Wang, Zhiming3; Tian, Leilei1

2022-09-01

10.1002/adfm.202207845

ADVANCED FUNCTIONAL MATERIALS   Impact Factor & Quartile

1616-301X

1616-3028

A nanomaterial with a high payload of nucleic acid drugs and low cytotoxicity is desirable for gene therapy. Herein, a new DNA assembly method triggered by hydrophobic interaction and strengthened by Mg2+ electrostatic interaction for carrier-free gene transfection is reported. It is discovered that a hydrophobic aggregation-induced emission dye with a low molecular weight that is modified to an oligonucleotide will greatly change the condensation properties of DNA. The conditions and the possible mechanism of such an aggregation process are carefully investigated. Further, it is revealed that the resultant aggregate is suitable for gene delivery, as in which the oligonucleotides are protected from degradation, and the intracellular permeability is enhanced. For the delivery of Bcl-2 antisense oligonucleotide, such a system exhibits a high gene transfection efficiency both in vitro and in vivo. Accordingly, an interesting observation is reported in this work that an aggregation-induced emission dye with a molecular weight much lower than the nucleic acid drugs can cause oligonucleotide aggregation and facilitate gene transfection. Such a delivery system provides a practical and inspiring example for the development of gene therapy in the future.

aggregation-induced emission antisense oligonucleotides DNA nanotechnology gene delivery hydrophobic interactions

SCI ; EI

English

NI Journal Papers

First ; Corresponding

National Natural Science Foundation of China["51973089","82101980"] ; Shenzhen Science and Technology Innovation Commission[KQTD20170810111314625]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000852765800001

WILEY-V C H VERLAG GMBH

20223812749435

Condensation ; Controlled drug delivery ; DNA ; Gene transfer ; Hydrophobicity ; Molecular weight ; Oligonucleotides ; Targeted drug delivery

Biological Materials and Tissue Engineering:461.2 ; Genetic Engineering:461.8.1 ; Biology:461.9 ; Chemical Operations:802.3 ; Physical Properties of Gases, Liquids and Solids:931.2 ; Atomic and Molecular Physics:931.3

MATERIALS SCIENCE

Web of Science

1.Southern Univ Sci & Technol, Dept Mat Sci & Engn, 1088 Xueyuan Blvd, Shenzhen 518055, Guangdong, Peoples R China
2.Shanxi Med Univ, Sch Forens Med, 56 Xinjian South Rd, Taiyuan 030001, Shanxi, Peoples R China
3.South China Univ Technol, Key Lab Luminescent Mat & Devices, Ctr Aggregat Induced Emiss, Key Lab Luminescence Mol Aggregates Guangdong Pro, Guangzhou Int Campus, Guangzhou 510640, Peoples R China

Chen, Zhe,Wei, Zixiang,Xiao, Fan,et al. The Hydrophobicity of AIE Dye Facilitates DNA Condensation for Carrier-Free Gene Therapy[J]. ADVANCED FUNCTIONAL MATERIALS,2022.

Chen, Zhe.,Wei, Zixiang.,Xiao, Fan.,Chao, Zhicong.,Lu, Jingxiong.,...&Tian, Leilei.(2022).The Hydrophobicity of AIE Dye Facilitates DNA Condensation for Carrier-Free Gene Therapy.ADVANCED FUNCTIONAL MATERIALS.

Chen, Zhe,et al."The Hydrophobicity of AIE Dye Facilitates DNA Condensation for Carrier-Free Gene Therapy".ADVANCED FUNCTIONAL MATERIALS (2022).