A MYOCARDIAL INFARCT BORDER-ZONE-ON-A-CHIP DEMONSTRATES AN OXYGEN GRADIENT REGULATES CARDIAC TISSUE FUNCTION

Rexius-Hall，Megan L.1; Khalil，Natalie N.1; Li，Xin2; Hu，Jiayi2; Yuan，Hongyan2; Escopete，Sean3; Parker，Sarah J.3; McCain，Megan L.1

2021

MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

103-104

After a myocardial infarction (MI), the border zone (BZ) is characterized by an oxygen (O2) gradient located at the boundary between the injured, hypoxic cardiac tissue and adjacent normoxic, viable tissue. In this study, underlying microfluidic channels were used to generate and expose engineered cardiac tissues to stable O2 gradients as a “Border-Zone-on-a-Chip” model. Functional and transcriptomic analysis revealed that calcium transients, calcium wave propagation velocity (CPV), contractile stress, and gene expression were uniquely regulated by an O2 gradient when compared to engineered tissues exposed to global, homogeneous O2 levels.

Cardiomyocytes Hypoxia Organs on Chip

Others

English

EI

National Institutes of Health[19POST34380814];National Institutes of Health[R01 HL153286-01];

20223512675354

Calcium ; Cardiology ; Gene expression ; Heart ; Histology ; Oxygen ; Wave propagation

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biology:461.9 ; Alkaline Earth Metals:549.2 ; Chemical Products Generally:804

2-s2.0-85136982499

Scopus

1.Laboratory for Living Systems Engineering,Department of Biomedical Engineering,USC Viterbi School of Engineering,University of Southern California,Los Angeles,United States
2.Department of Mechanics and Aerospace Engineering,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
3.Smidt Heart Institute,Cedars-Sinai Medical Center,Los Angeles,United States

Rexius-Hall，Megan L.,Khalil，Natalie N.,Li，Xin,et al. A MYOCARDIAL INFARCT BORDER-ZONE-ON-A-CHIP DEMONSTRATES AN OXYGEN GRADIENT REGULATES CARDIAC TISSUE FUNCTION[C],2021:103-104.