CPSF30-L-mediated recognition of mRNA modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis

2021

Molecular Plant   Impact Factor & Quartile

1674-2052

N6-methyladenosine (m6A),a ubiquitous internal modification of eukaryotic mRNAs,plays a vital role in almost every aspect of mRNA metabolism.However,there is little evidence documenting the role of m6A in regulating alternative polyadenylation (APA) in plants.APA is controlled by a large protein-RNA complex with many components,including CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30 (CPSF30).In Arabidopsis,CPSF30 has two isoforms and the longer isoform (CPSF30-L) contains a YT512-B Homology (YTH) domain,which is unique to plants.In this study,we showed that CPSF30-L YTH domain binds to m6A in vitro.In the cpsf30-2 mutant,the transcripts of many genes including several important nitrate signaling-related genes had shifts in polyadenylation sites that were correlated with m6A peaks,indicating that these gene transcripts carrying m6A tend to be regulated by APA.Wild-type CPSF30-L could rescue the defects in APA and nitrate metabolism in cpsf30-2,but m6A-binding-defective mutants of CPSF30-L could not.Taken together,our results demonstrated that m6A modification regulates APA in Arabidopsis and revealed that the m6A reader CPSF30-L affects nitrate signaling by controlling APA,shedding new light on the roles of the m6A modification during RNA 3'-end processing in nitrate metabolism.

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WanFang

fzzw-e202104019

1.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
2.Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China;Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, RNA Biomedical Institute, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China
3.State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China
4.Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China
5.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China;Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Beijing 100101, China

. CPSF30-L-mediated recognition of mRNA modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis[J]. Molecular Plant,2021,14(4):688-699.

(2021).CPSF30-L-mediated recognition of mRNA modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis.Molecular Plant,14(4),688-699.

"CPSF30-L-mediated recognition of mRNA modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis".Molecular Plant 14.4(2021):688-699.