中文版 | English
Title

Regulation of cohesin-mediated chromosome folding by PDS5 in mammals

Author
Corresponding AuthorYu, Dingdang; Cai, Jiabin; Chen, Hao
Publication Years
2022-09-01
DOI
Source Title
ISSN
1469-221X
EISSN
1469-3178
Abstract
Cohesin regulates sister chromatid cohesion but also contributes to chromosome folding by promoting the formation of chromatin loops, a process mediated by loop extrusion. Although PDS5 regulates cohesin dynamics on chromatin, the exact function of PDS5 in cohesin-mediated chromatin looping remains unclear. Two paralogs of PDS5 exist in vertebrates, PDS5A and PDS5B. Here we show that PDS5A and PDS5B co-localize with RAD21 and CTCF at loop anchors. Rapid PDS5A or PDS5B degradation in liver cancer cells using an inducible degron system reduces chromatin loops and increases loop size. RAD21 enrichment at loop anchors is decreased upon depletion of PDS5A or PDS5B. PDS5B loss also reduces CTCF signals at loop anchors and has a stronger effect on loop enlargement compared with PDS5A. Co-depletion of PDS5A and PDS5B reduces RAD21 levels at loop anchors although the amount of cohesin on chromatin is increased. Our study provides insight into how PDS5 proteins regulate cohesin-mediated chromatin looping.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
Funding Project
National Natural Science Foundation of China[32170604] ; National Key Research and Development Program of China[2018YFA0108700]
WOS Research Area
Biochemistry & Molecular Biology ; Cell Biology
WOS Subject
Biochemistry & Molecular Biology ; Cell Biology
WOS Accession No
WOS:000855896500001
Publisher
ESI Research Field
MOLECULAR BIOLOGY & GENETICS
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/402373
DepartmentSchool of Medicine
Affiliation
1.Fudan Univ, Inst Biomed Sci, Key Lab Med Epigenet & Metab, Shanghai, Peoples R China
2.Southern Univ Sci & Technol, Sch Med, Dept Human Cell Biol & Genet, Shenzhen, Peoples R China
3.Renji Hosp, Shanghai Canc Inst, State Key Lab Oncogenes & Related Genes, Shanghai, Peoples R China
4.Shanghai Jiao Tong Univ, Ren Ji Hosp, Renji Med X Clin Stem Cell Res Ctr, Sch Med, Shanghai, Peoples R China
5.Fudan Univ, Zhongshan Hosp, Liver Canc Inst, Minist Educ,Key Lab Carcinogenesis & Canc Invas, Shanghai, Peoples R China
6.Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Guangdong Cardiovasc Inst, Dept Cardiac Surg, Guangzhou, Peoples R China
7.Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Guangdong Cardiovasc Inst, Dept Med Sci, Guangzhou, Peoples R China
First Author AffilicationSchool of Medicine
Corresponding Author AffilicationSchool of Medicine
Recommended Citation
GB/T 7714
Yu, Dingdang,Chen, Guoyu,Wang, Yuci,et al. Regulation of cohesin-mediated chromosome folding by PDS5 in mammals[J]. EMBO REPORTS,2022.
APA
Yu, Dingdang.,Chen, Guoyu.,Wang, Yuci.,Wang, Yining.,Lin, Risheng.,...&Chen, Hao.(2022).Regulation of cohesin-mediated chromosome folding by PDS5 in mammals.EMBO REPORTS.
MLA
Yu, Dingdang,et al."Regulation of cohesin-mediated chromosome folding by PDS5 in mammals".EMBO REPORTS (2022).
Files in This Item:
There are no files associated with this item.
Related Services
Fulltext link
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Export to Excel
Export to Csv
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Yu, Dingdang]'s Articles
[Chen, Guoyu]'s Articles
[Wang, Yuci]'s Articles
Baidu Scholar
Similar articles in Baidu Scholar
[Yu, Dingdang]'s Articles
[Chen, Guoyu]'s Articles
[Wang, Yuci]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yu, Dingdang]'s Articles
[Chen, Guoyu]'s Articles
[Wang, Yuci]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.