中文版 | English
Title

ISSAAC-seq enables sensitive and flexible multimodal profiling of chromatin accessibility and gene expression in single cells

Author
Corresponding AuthorJin, Wenfei; Chen, Xi
Joint first authorXu, Wei; Yang, Weilong
Publication Years
2022-09-01
DOI
Source Title
ISSN
1548-7091
EISSN
1548-7105
Abstract

["Joint profiling of chromatin accessibility and gene expression from the same single cell provides critical information about cell types in a tissue and cell states during a dynamic process. Here, we develop in situ sequencing hetero RNA-DNA-hybrid after assay for transposase-accessible chromatin-sequencing (ISSAAC-seq), a highly sensitive and flexible single-cell multi-omics method to interrogate chromatin accessibility and gene expression from the same single nucleus. We demonstrated that ISSAAC-seq is sensitive and provides high quality data with orders of magnitude more features than existing methods. Using the joint profiles from over 10,000 nuclei from the mouse cerebral cortex, we uncovered major and rare cell types and cell-type specific regulatory elements and identified heterogeneity at the chromatin level within established cell types defined by gene expression. Finally, we revealed distinct dynamics and relationships of gene expression and chromatin accessibility during an oligodendrocyte maturation trajectory.","ISSAAC-seq offers a sensitive and modular tool for joint profiling gene expression and chromatin accessibility in single cells."]

URL[Source Record]
Indexed By
Language
English
Important Publications
NI Journal Papers
SUSTech Authorship
First ; 共同第一 ; Corresponding
Funding Project
National Key R&D Program of China[2021YFF1200900] ; National Natural Science Foundation of China[
WOS Research Area
Biochemistry & Molecular Biology
WOS Subject
Biochemical Research Methods
WOS Accession No
WOS:000854718900002
Publisher
ESI Research Field
BIOLOGY & BIOCHEMISTRY
Data Source
Web of Science
Publication Status
正式出版
Citation statistics
Cited Times [WOS]:5
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/402378
DepartmentDepartment of Biology
生命科学学院
Affiliation
1.Southern Univ Sci & Technol, Sch Life Sci, Dept Biol, Shenzhen Key Lab Gene Regulat & Syst Biol, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Brain Res Ctr, Shenzhen, Peoples R China
3.Southern Univ Sci & Technol, Dept Biol, Sch Life Sci, Shenzhen, Peoples R China
First Author AffilicationDepartment of Biology;  School of Life Sciences
Corresponding Author AffilicationDepartment of Biology;  School of Life Sciences
First Author's First AffilicationDepartment of Biology;  School of Life Sciences
Recommended Citation
GB/T 7714
Xu, Wei,Yang, Weilong,Zhang, Yunlong,et al. ISSAAC-seq enables sensitive and flexible multimodal profiling of chromatin accessibility and gene expression in single cells[J]. NATURE METHODS,2022.
APA
Xu, Wei.,Yang, Weilong.,Zhang, Yunlong.,Chen, Yawen.,Hong, Ni.,...&Chen, Xi.(2022).ISSAAC-seq enables sensitive and flexible multimodal profiling of chromatin accessibility and gene expression in single cells.NATURE METHODS.
MLA
Xu, Wei,et al."ISSAAC-seq enables sensitive and flexible multimodal profiling of chromatin accessibility and gene expression in single cells".NATURE METHODS (2022).
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