A key F27I substitution within HCDR1 facilitates the rapid maturation of P2C-1F11-like neutralizing antibodies in a SARS-CoV-2-infected donor

Wang，Miao1; Fan，Qing1; Zhou，Bing1; Ye，Haocheng1; Shen，Senlin1; Yu，Jiazhen1; Cheng，Lin1; Ge，Xiangyang1; Ju，Bin1,2; Zhang，Zheng1,2,3

2022-09-13

10.1016/j.celrep.2022.111335

Cell Reports   Impact Factor & Quartile

2211-1247

Although thousands of anti-SARS-CoV-2 monoclonal neutralizing antibodies (nAbs) have been identified and well characterized, some crucial events in the development of these nAbs during viral infection remain unclear. Using deep sequencing, we explore the dynamics of antibody repertoire in a SARS-CoV-2-infected donor, from whom the potent and broad nAb P2C-1F11 (the parent version of Brii-196) was previously isolated. Further analysis shows a rapid clonal expansion of some SARS-CoV-2-specific antibodies in early infection. Longitudinal tracing of P2C-1F11 lineage antibodies reveals that these elite nAbs were rare. Using sequence alignment, structure modeling, and bioactivity analysis based on site-mutated assay, we demonstrate that a key substitution F27I in heavy chain contributes significantly to the maturation of P2C-1F11-like antibodies. Overall, our findings elucidate the developmental process and maturation pathway of P2C-1F11, providing some important information for the design of novel immunogens to elicit more potent nAbs against SARS-CoV-2 infection.

antibody repertoire CP: Immunology deep sequencing neutralizing antibody P2C-1F11 lineage SARS-CoV-2

English

First ; Corresponding

Basic and Applied Basic Research Foundation of Guangdong Province[2019A1515011197];Basic and Applied Basic Research Foundation of Guangdong Province[2020A1515110656];Basic and Applied Basic Research Foundation of Guangdong Province[2021A1515011009];Basic and Applied Basic Research Foundation of Guangdong Province[2021B1515020034];China Postdoctoral Science Foundation[2021M703445];National Natural Science Foundation of China[82002140];National Natural Science Foundation of China[82101861];National Natural Science Foundation of China[82171752];National Natural Science Foundation of China[92169204];Natural Science Foundation of Shenzhen City[JCYJ20190809115617365];Natural Science Foundation of Shenzhen City[JCYJ20210324115611032];Natural Science Foundation of Shenzhen City[JCYJ20210324131606018];

2-s2.0-85137776040

Scopus

1.Institute for Hepatology,National Clinical Research Center for Infectious Disease,Shenzhen Third People's Hospital; the Second Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong Province,518112,China
2.Guangdong Key Laboratory for Anti-infection Drug Quality Evaluation,Shenzhen,Guangdong Province,518112,China
3.Shenzhen Research Center for Communicable Disease Diagnosis and Treatment of Chinese Academy of Medical Science,Shenzhen,Guangdong Province,518112,China

Wang，Miao,Fan，Qing,Zhou，Bing,et al. A key F27I substitution within HCDR1 facilitates the rapid maturation of P2C-1F11-like neutralizing antibodies in a SARS-CoV-2-infected donor[J]. Cell Reports,2022,40(11).

Wang，Miao.,Fan，Qing.,Zhou，Bing.,Ye，Haocheng.,Shen，Senlin.,...&Zhang，Zheng.(2022).A key F27I substitution within HCDR1 facilitates the rapid maturation of P2C-1F11-like neutralizing antibodies in a SARS-CoV-2-infected donor.Cell Reports,40(11).

Wang，Miao,et al."A key F27I substitution within HCDR1 facilitates the rapid maturation of P2C-1F11-like neutralizing antibodies in a SARS-CoV-2-infected donor".Cell Reports 40.11(2022).