Mechanical Stabilization of a Bacterial Adhesion Complex
The adhesions between Gram-positive bacteria and their hosts are exposed to varying magnitudes of tensile forces. Here, using an ultrastable magnetic tweezer-based single-molecule approach, we show the catch-bond kinetics of the prototypical adhesion complex of SD-repeat protein G (SdrG) to a peptide from fibrinogen β (Fgβ) over a physiologically important force range from piconewton (pN) to tens of pN, which was not technologically accessible to previous studies. At 37 °C, the lifetime of the complex exponentially increases from seconds at several pN to ∼1000 s as the force reaches 30 pN, leading to mechanical stabilization of the adhesion. The dissociation transition pathway is determined as the unbinding of a critical β-strand peptide ("latch"strand of SdrG that secures the entire adhesion complex) away from its binding cleft, leading to the dissociation of the Fgβ ligand. Similar mechanical stabilization behavior is also observed in several homologous adhesions, suggesting the generality of catch-bond kinetics in such bacterial adhesions. We reason that such mechanical stabilization confers multiple advantages in the pathogenesis and adaptation of bacteria.
NI Journal Papers
Singapore Ministry of Education Academic Research Fund Tier 2[
|WOS Research Area|
|WOS Accession No|
|EI Accession Number|
Bacteria ; Dissociation ; Peptides ; Stabilization
|ESI Classification Code|
Biology:461.9 ; Chemical Reactions:802.2 ; Materials Science:951
Cited Times [WOS]:3
|Document Type||Journal Article|
|Department||Department of Biomedical Engineering|
1.Department of Physics,National University of Singapore,Singapore,117542,Singapore
2.Mechanobiology Institute,National University of Singapore,Singapore,117411,Singapore
3.Centre for Bioimaging Sciences,National University of Singapore,Singapore,117546,Singapore
4.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
5.Institute of Materials Research and Engineering,A∗STAR,Singapore,2 Fusionopolis Way, Innovis, #08-03,138634,Singapore
6.Research Institute for Biomimetics and Soft Matter,Fujian Provincial Key Lab for Soft Functional Materials Research,Department of Physics,Xiamen University,Xiamen,361005,China
Huang，Wenmao,Le，Shimin,Sun，Yuze,et al. Mechanical Stabilization of a Bacterial Adhesion Complex[J]. Journal of the American Chemical Society,2022.
Huang，Wenmao.,Le，Shimin.,Sun，Yuze.,Lin，Dennis Jingxiong.,Yao，Mingxi.,...&Yan，Jie.(2022).Mechanical Stabilization of a Bacterial Adhesion Complex.Journal of the American Chemical Society.
Huang，Wenmao,et al."Mechanical Stabilization of a Bacterial Adhesion Complex".Journal of the American Chemical Society (2022).
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