Derivatization strategy for sensitive identiﬁcation of neutral and acidic glycosphingolipids using RPLC-MS
Glycosphingolipids are essential components of the cell membrane and implicate in a myriad of diseases. Glycosphingolipids are classified as neutral and acidic families based on the glycan structure. Neutral glycosphingolipids possess one or more neutral monosaccharide units, while acidic glycosphingolipids (such as gangliosides) contain one or more sialic acid (classified as NeuAc and NeuGc) linking on the glycan part. To better understand the metabolism process of glycosphingolipids, simultaneous identification of neutral and acidic glycosphingolipids is often required. However, it is hard to analyze gangliosides in positive-ion mode due to its poor ionization efficiency. Herein, we proposed a new strategy based on chemical derivatization to stabilize sialic acid and improve MS detection sensitivity of gangliosides in positive-ion mode. The carboxyl group of sialic acid were labeled with N,N-dimethylethylenediamine (DMEN), which contains a positively ionizable tertiary amine group. The labeling efficiency of DMEN was close to 100% and the ionization eﬃciency of gangliosides was enhanced for more than 4-fold. The fragments of DMEN labeled gangliosides provide a diagnosis ion to facilitate rapid structural assignments of gangliosides, and also help discrimination of isomeric gangliosides with different sialic acid location. By using DMEN derivatization, 45 glycosphingolipids were identified from human plasma, including 30 gangliosides and 15 neutral glycosphingolipids. Our strategy could therefore be a generic approach for simultaneous MS identification of neutral and acidic glycosphingolipids.
First ; Corresponding
China Postdoctoral Science Foundation[2020M683168] ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Southern University of Science and Technology[G02216304/G02216404]
|WOS Research Area|
Physics ; Spectroscopy
Physics, Atomic, Molecular & Chemical ; Spectroscopy
|WOS Accession No|
|ESI Research Field|
Cited Times [WOS]:0
|Document Type||Journal Article|
|Department||Department of Chemistry|
1.Department of Chemistry and Research Center for Chemical Biology and Omics Analysis,Southern University of Science and Technology,Shenzhen,518055,China
2.Department of Oncology,The Second Clinical Medical College,Jinan University (Shenzhen People's Hospital),Shenzhen,518020,China
3.The First Affiliated Hospital,Jinan University,Guangzhou,510632,China
4.SUSTech Core Research Facilities,Southern University of Science and Technology,Shenzhen,518055,China
|First Author Affilication||Department of Chemistry|
|Corresponding Author Affilication||Department of Chemistry|
|First Author's First Affilication||Department of Chemistry|
Liu，Yilian,Yang，Lijun,Li，Hua,et al. Derivatization strategy for sensitive identiﬁcation of neutral and acidic glycosphingolipids using RPLC-MS[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2022,482:116937.
Liu，Yilian,Yang，Lijun,Li，Hua,Liu，Jie,&Tian，Ruijun.(2022).Derivatization strategy for sensitive identiﬁcation of neutral and acidic glycosphingolipids using RPLC-MS.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,482,116937.
Liu，Yilian,et al."Derivatization strategy for sensitive identiﬁcation of neutral and acidic glycosphingolipids using RPLC-MS".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 482(2022):116937.
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