中文版 | English
Title

Derivatization strategy for sensitive identification of neutral and acidic glycosphingolipids using RPLC-MS

Author
Corresponding AuthorTian,Ruijun
Publication Years
2022-12-01
DOI
Source Title
ISSN
1387-3806
EISSN
1873-2798
Volume482Pages:116937
Abstract

Glycosphingolipids are essential components of the cell membrane and implicate in a myriad of diseases. Glycosphingolipids are classified as neutral and acidic families based on the glycan structure. Neutral glycosphingolipids possess one or more neutral monosaccharide units, while acidic glycosphingolipids (such as gangliosides) contain one or more sialic acid (classified as NeuAc and NeuGc) linking on the glycan part. To better understand the metabolism process of glycosphingolipids, simultaneous identification of neutral and acidic glycosphingolipids is often required. However, it is hard to analyze gangliosides in positive-ion mode due to its poor ionization efficiency. Herein, we proposed a new strategy based on chemical derivatization to stabilize sialic acid and improve MS detection sensitivity of gangliosides in positive-ion mode. The carboxyl group of sialic acid were labeled with N,N-dimethylethylenediamine (DMEN), which contains a positively ionizable tertiary amine group. The labeling efficiency of DMEN was close to 100% and the ionization efficiency of gangliosides was enhanced for more than 4-fold. The fragments of DMEN labeled gangliosides provide a diagnosis ion to facilitate rapid structural assignments of gangliosides, and also help discrimination of isomeric gangliosides with different sialic acid location. By using DMEN derivatization, 45 glycosphingolipids were identified from human plasma, including 30 gangliosides and 15 neutral glycosphingolipids. Our strategy could therefore be a generic approach for simultaneous MS identification of neutral and acidic glycosphingolipids.

Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
First ; Corresponding
Funding Project
China Postdoctoral Science Foundation[2020M683168] ; National Natural Science Foundation of China[22004049] ; National Natural Science Foundation of China[31700088] ; National Natural Science Foundation of China[32171433] ; Southern University of Science and Technology[G02216304/G02216404]
WOS Research Area
Physics ; Spectroscopy
WOS Subject
Physics, Atomic, Molecular & Chemical ; Spectroscopy
WOS Accession No
WOS:000862686900004
Publisher
ESI Research Field
CHEMISTRY
Scopus EID
2-s2.0-85138588064
Data Source
Scopus
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/402637
DepartmentDepartment of Chemistry
公共分析测试中心
Affiliation
1.Department of Chemistry and Research Center for Chemical Biology and Omics Analysis,Southern University of Science and Technology,Shenzhen,518055,China
2.Department of Oncology,The Second Clinical Medical College,Jinan University (Shenzhen People's Hospital),Shenzhen,518020,China
3.The First Affiliated Hospital,Jinan University,Guangzhou,510632,China
4.SUSTech Core Research Facilities,Southern University of Science and Technology,Shenzhen,518055,China
First Author AffilicationDepartment of Chemistry
Corresponding Author AffilicationDepartment of Chemistry
First Author's First AffilicationDepartment of Chemistry
Recommended Citation
GB/T 7714
Liu,Yilian,Yang,Lijun,Li,Hua,et al. Derivatization strategy for sensitive identification of neutral and acidic glycosphingolipids using RPLC-MS[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2022,482:116937.
APA
Liu,Yilian,Yang,Lijun,Li,Hua,Liu,Jie,&Tian,Ruijun.(2022).Derivatization strategy for sensitive identification of neutral and acidic glycosphingolipids using RPLC-MS.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,482,116937.
MLA
Liu,Yilian,et al."Derivatization strategy for sensitive identification of neutral and acidic glycosphingolipids using RPLC-MS".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 482(2022):116937.
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