Title | Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism |
Author | |
Publication Years | 2022-09-09
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DOI | |
Source Title | |
ISSN | 2375-2548
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EISSN | 2375-2548
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Volume | 8Issue:36 |
Abstract | Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. The direct recognition mechanism of pathogen effectors by coiled-coil NLRs (CNLs) remains unclear. We demonstrate that the Triticum monococcum CNL Sr35 directly recognizes the pathogen effector AvrSr35 from Puccinia graminis f. sp. tritici and report a cryo-electron microscopy structure of Sr35 resistosome and a crystal structure of AvrSr35. We show that AvrSr35 forms homodimers that are disassociated into monomers upon direct recognition by the leucine-rich repeat domain of Sr35, which induces Sr35 resistosome assembly and the subsequent immune response. The first 20 amino-terminal residues of Sr35 are indispensable for immune signaling but not for plasma membrane association. Our findings reveal the direct recognition and activation mechanism of a plant CNL and provide insights into biochemical function of Sr35 resistosome. |
URL | [Source Record] |
Indexed By | |
Language | English
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Important Publications | NI Journal Papers
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SUSTech Authorship | Others
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Funding Project | National Key Research and Development Program of China[2021YFC2301403]
; National Natural Science Foundation of China["82225028","82172287"]
; Chinese Academy of Sciences[SKF2020NO1]
; China Postdoctoral Science Foundation[2020 M682067]
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WOS Research Area | Science & Technology - Other Topics
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WOS Subject | Multidisciplinary Sciences
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WOS Accession No | WOS:000911968500032
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Publisher | |
Data Source | Web of Science
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Citation statistics |
Cited Times [WOS]:16
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Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/402696 |
Department | Cryo-Electron Microscopy Center 南方科技大学 |
Affiliation | 1.Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation,Key Laboratory of Innate Immune Biology of Fujian Province,Biomedical Research Center of South China,Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education,College of Life Sciences,Fujian Normal University,Fuzhou,350117,China 2.Cryo-EM Centre,Southern University of Science and Technology,Shenzhen,515055,China 3.Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,Plant Immunity Center,Fujian Agriculture and Forestry University,Fuzhou,350002,China |
Recommended Citation GB/T 7714 |
Zhao,Yan Bo,Liu,Meng Xi,Chen,Tao Tao,et al. Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism[J]. Science advances,2022,8(36).
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APA |
Zhao,Yan Bo.,Liu,Meng Xi.,Chen,Tao Tao.,Ma,Xiaomin.,Li,Ze Kai.,...&Ouyang,Songying.(2022).Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism.Science advances,8(36).
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MLA |
Zhao,Yan Bo,et al."Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism".Science advances 8.36(2022).
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