中文版 | English
Title

Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism

Author
Publication Years
2022-09-09
DOI
Source Title
ISSN
2375-2548
EISSN
2375-2548
Volume8Issue:36
Abstract

Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. The direct recognition mechanism of pathogen effectors by coiled-coil NLRs (CNLs) remains unclear. We demonstrate that the Triticum monococcum CNL Sr35 directly recognizes the pathogen effector AvrSr35 from Puccinia graminis f. sp. tritici and report a cryo-electron microscopy structure of Sr35 resistosome and a crystal structure of AvrSr35. We show that AvrSr35 forms homodimers that are disassociated into monomers upon direct recognition by the leucine-rich repeat domain of Sr35, which induces Sr35 resistosome assembly and the subsequent immune response. The first 20 amino-terminal residues of Sr35 are indispensable for immune signaling but not for plasma membrane association. Our findings reveal the direct recognition and activation mechanism of a plant CNL and provide insights into biochemical function of Sr35 resistosome.

URL[Source Record]
Indexed By
Language
English
Important Publications
NI Journal Papers
SUSTech Authorship
Others
Funding Project
National Key Research and Development Program of China[2021YFC2301403] ; National Natural Science Foundation of China["82225028","82172287"] ; Chinese Academy of Sciences[SKF2020NO1] ; China Postdoctoral Science Foundation[2020 M682067]
WOS Research Area
Science & Technology - Other Topics
WOS Subject
Multidisciplinary Sciences
WOS Accession No
WOS:000911968500032
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:9
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/402696
DepartmentCryo-Electron Microscopy Center
南方科技大学
Affiliation
1.Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation,Key Laboratory of Innate Immune Biology of Fujian Province,Biomedical Research Center of South China,Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education,College of Life Sciences,Fujian Normal University,Fuzhou,350117,China
2.Cryo-EM Centre,Southern University of Science and Technology,Shenzhen,515055,China
3.Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,Plant Immunity Center,Fujian Agriculture and Forestry University,Fuzhou,350002,China
Recommended Citation
GB/T 7714
Zhao,Yan Bo,Liu,Meng Xi,Chen,Tao Tao,et al. Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism[J]. Science advances,2022,8(36).
APA
Zhao,Yan Bo.,Liu,Meng Xi.,Chen,Tao Tao.,Ma,Xiaomin.,Li,Ze Kai.,...&Ouyang,Songying.(2022).Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism.Science advances,8(36).
MLA
Zhao,Yan Bo,et al."Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism".Science advances 8.36(2022).
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