Inhibition of CCR8 attenuates Ang Ⅱ-induced vascular smooth muscle cell injury by suppressing the MAPK/NF-κB pathway

Liao，Lisi1; Song，Di1; Shi，Bobo1; Chen，Ming1; Wu，Linghu1; Xu，Jinfeng1,2; Dong，Fajin1,2

2022-09-01

10.22038/IJBMS.2022.64524.14191

Iranian Journal of Basic Medical Sciences   Impact Factor & Quartile

2008-3866

2008-3874

Objective(s): Hyperinsulinemia, secondary to insulin resistance, may lead to vascular smooth muscle cell dysfunction. In the present research, we aimed to investigate the effect of Chemokine receptor 8 (CCR8) on angiotensin II (Ang II)-induced dysfunction of vascular smooth muscle cells (VSMCs) and to explore the underlying molecular mechanism. Materials and Methods: The expression of CCR8 was analyzed in diabetics and normal people by RT-PCR and ELISA. CCK-8 assay and transwell were used to explore cell proliferation and migration, and ELISA was used to measure the content of IL-6 and TNF-α. Reactive oxygen species (ROS) kit was employed to measure ROS generation. Results: The results revealed that CCR8 was highly expressed in diabetics and Ang Ⅱ-induced VSMCs. Further studies found that interfering with the expression of CCR8 significantly reduced the production of ROS and the levels of inflammatory factors in AngⅡ-induced VSMCs. Interfering with CCR8 increased the glucose uptake induced by AngⅡ+IR. More importantly, inhibition of CCR8 alleviated Ang II-induced dysfunction of VSMCs. Inhibition of CCR8 inactivated the MAPK/NF-κB signaling pathway. Conclusion: Inhibition of CCR8 attenuates Ang II-induced VSMCs injury by inhibiting the MAPK/NFκB pathway. CCR8 may be a new biomarker related to hypertension and insulin resistance and is a new target for the treatment of human cardiovascular diseases.

CCR8 Hypertension Inflammation Insulin resistance MAPK/NF-κB signaling Oxidative

English

First ; Corresponding

2-s2.0-85138633140

Scopus

1.The Second Clinical Medical College,Jinan University,Department of Ultrasound,The First Affiliated Hospital,Southern University of Science and Technology,Department of Ultrasound,Shenzhen People’s Hospital,Shenzhen,Guangdong,518020,China
2.

Liao，Lisi,Song，Di,Shi，Bobo,et al. Inhibition of CCR8 attenuates Ang Ⅱ-induced vascular smooth muscle cell injury by suppressing the MAPK/NF-κB pathway[J]. Iranian Journal of Basic Medical Sciences,2022,26(9):1097-1103.

Liao，Lisi.,Song，Di.,Shi，Bobo.,Chen，Ming.,Wu，Linghu.,...&Dong，Fajin.(2022).Inhibition of CCR8 attenuates Ang Ⅱ-induced vascular smooth muscle cell injury by suppressing the MAPK/NF-κB pathway.Iranian Journal of Basic Medical Sciences,26(9),1097-1103.

Liao，Lisi,et al."Inhibition of CCR8 attenuates Ang Ⅱ-induced vascular smooth muscle cell injury by suppressing the MAPK/NF-κB pathway".Iranian Journal of Basic Medical Sciences 26.9(2022):1097-1103.