中文版 | English
Title

Inhibition of CCR8 attenuates Ang Ⅱ-induced vascular smooth muscle cell injury by suppressing the MAPK/NF-κB pathway

Author
Corresponding AuthorXu,Jinfeng
Publication Years
2022-09-01
DOI
Source Title
ISSN
2008-3866
EISSN
2008-3874
Volume26Issue:9Pages:1097-1103
Abstract
Objective(s): Hyperinsulinemia, secondary to insulin resistance, may lead to vascular smooth muscle cell dysfunction. In the present research, we aimed to investigate the effect of Chemokine receptor 8 (CCR8) on angiotensin II (Ang II)-induced dysfunction of vascular smooth muscle cells (VSMCs) and to explore the underlying molecular mechanism. Materials and Methods: The expression of CCR8 was analyzed in diabetics and normal people by RT-PCR and ELISA. CCK-8 assay and transwell were used to explore cell proliferation and migration, and ELISA was used to measure the content of IL-6 and TNF-α. Reactive oxygen species (ROS) kit was employed to measure ROS generation. Results: The results revealed that CCR8 was highly expressed in diabetics and Ang Ⅱ-induced VSMCs. Further studies found that interfering with the expression of CCR8 significantly reduced the production of ROS and the levels of inflammatory factors in AngⅡ-induced VSMCs. Interfering with CCR8 increased the glucose uptake induced by AngⅡ+IR. More importantly, inhibition of CCR8 alleviated Ang II-induced dysfunction of VSMCs. Inhibition of CCR8 inactivated the MAPK/NF-κB signaling pathway. Conclusion: Inhibition of CCR8 attenuates Ang II-induced VSMCs injury by inhibiting the MAPK/NFκB pathway. CCR8 may be a new biomarker related to hypertension and insulin resistance and is a new target for the treatment of human cardiovascular diseases.
Keywords
URL[Source Record]
Language
English
SUSTech Authorship
First ; Corresponding
Scopus EID
2-s2.0-85138633140
Data Source
Scopus
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/402715
DepartmentShenzhen People's Hospital
Affiliation
1.The Second Clinical Medical College,Jinan University,Department of Ultrasound,The First Affiliated Hospital,Southern University of Science and Technology,Department of Ultrasound,Shenzhen People’s Hospital,Shenzhen,Guangdong,518020,China
2.
First Author AffilicationShenzhen People's Hospital
Corresponding Author AffilicationShenzhen People's Hospital
First Author's First AffilicationShenzhen People's Hospital
Recommended Citation
GB/T 7714
Liao,Lisi,Song,Di,Shi,Bobo,et al. Inhibition of CCR8 attenuates Ang Ⅱ-induced vascular smooth muscle cell injury by suppressing the MAPK/NF-κB pathway[J]. Iranian Journal of Basic Medical Sciences,2022,26(9):1097-1103.
APA
Liao,Lisi.,Song,Di.,Shi,Bobo.,Chen,Ming.,Wu,Linghu.,...&Dong,Fajin.(2022).Inhibition of CCR8 attenuates Ang Ⅱ-induced vascular smooth muscle cell injury by suppressing the MAPK/NF-κB pathway.Iranian Journal of Basic Medical Sciences,26(9),1097-1103.
MLA
Liao,Lisi,et al."Inhibition of CCR8 attenuates Ang Ⅱ-induced vascular smooth muscle cell injury by suppressing the MAPK/NF-κB pathway".Iranian Journal of Basic Medical Sciences 26.9(2022):1097-1103.
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