中文版 | English
Title

Chemoenzymatic Synthesis of SARS-CoV-2 Homogeneous O-Linked Glycopeptides for Exploring Their Inhibition Functions

Author
Corresponding AuthorWu,Zhigang; He,Yunjiao; Kong,Yun
Publication Years
2022
DOI
Source Title
ISSN
2373-8227
EISSN
2373-8227
Volume8Issue:10
Abstract
Harnessing highly conserved peptides derived from the receptor binding domain (RBD) of spike (S) protein to construct peptide-based inhibitors is one of the most effective strategies to fight against the ever-mutating coronavirus SARS-CoV-2. But how the O-glycosylation affects their inhibition abilities has not been intensively explored. Herein, an intrinsic O-glycosylated peptide P320-334 derived from RBD was screened and homogeneous O-linked glycopeptides containing Tn (GalNAcα1-O-Ser/Thr), T (Galβ1-3GalNAcα1-O-Ser/Thr), sialyl-Tn (sTn, Siaα2-6GalNAcα1-O-Ser/Thr), and sialyl-T (sT, Siaα2-3Galβ1-3GalNAcα1-O-Ser/Thr) structures were first synthesized via chemoenzymatic strategies. Compared with the unglycosylated peptide, the binding of sT-P320-334 to hACE2 was enhanced to 133% and the inhibition capacity against RBD-hACE2 binding of sTn- and sT-P320-334 was significantly increased up to 150-410%. Thus, our results suggest the sialic acid residue on the terminal of short O-glycan structures might strengthen the inhibition capacities of these peptide-based inhibitors, which might provide novel optimization directions for the inhibitor design.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
Funding Project
Shandong Province Key Research and Develop Program["2019 GSF 107048","2020CXGC010601"] ; National Natural Science Foundation of China["31500648","31971213"] ; Guangdong Basic, Applied Basic Research Foundation[2019A1515010647] ; Open Projects Fund of Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University[2019CCG08] ; Shenzhen Science and Technology Program[KQTD20200909113758004] ; Hebei Provincial Department of Human Resources and Social Security Grants["C201812","E2018100010"] ; Foundation of Hebei Educational Committee[QN2019217] ; Natural Science Foundation of Hebei Province[H2021208001]
WOS Research Area
Pharmacology & Pharmacy ; Infectious Diseases
WOS Subject
Chemistry, Medicinal ; Infectious Diseases
WOS Accession No
WOS:000859282900001
Publisher
Scopus EID
2-s2.0-85138766314
Data Source
Scopus
Citation statistics
Cited Times [WOS]:2
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/402752
DepartmentSchool of Medicine
Affiliation
1.National Glycoengineering Research Center,Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology,State Key Laboratory of Microbial Technology,Shandong University,Qingdao,266237,China
2.School of Medicine,Southern University of Science and Technology,Shenzhen,518055,China
3.School of Health and Life Sciences,University of Health and Rehabilitation Sciences,Qingdao,266071,China
4.College of Food and Biology,Hebei University of Science and Technology,Shijiazhuang,050018,China
Corresponding Author AffilicationSchool of Medicine
Recommended Citation
GB/T 7714
Rong,Yongheng,Wang,Xingyun,Mao,Weian,et al. Chemoenzymatic Synthesis of SARS-CoV-2 Homogeneous O-Linked Glycopeptides for Exploring Their Inhibition Functions[J]. ACS Infectious Diseases,2022,8(10).
APA
Rong,Yongheng.,Wang,Xingyun.,Mao,Weian.,Yuan,Fang.,Chen,Min.,...&Kong,Yun.(2022).Chemoenzymatic Synthesis of SARS-CoV-2 Homogeneous O-Linked Glycopeptides for Exploring Their Inhibition Functions.ACS Infectious Diseases,8(10).
MLA
Rong,Yongheng,et al."Chemoenzymatic Synthesis of SARS-CoV-2 Homogeneous O-Linked Glycopeptides for Exploring Their Inhibition Functions".ACS Infectious Diseases 8.10(2022).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Export to Excel
Export to Csv
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Rong,Yongheng]'s Articles
[Wang,Xingyun]'s Articles
[Mao,Weian]'s Articles
Baidu Scholar
Similar articles in Baidu Scholar
[Rong,Yongheng]'s Articles
[Wang,Xingyun]'s Articles
[Mao,Weian]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Rong,Yongheng]'s Articles
[Wang,Xingyun]'s Articles
[Mao,Weian]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.