COP1 SUPPRESSOR 6 represses the PIF4 and PIF5 action to promote light-inhibited hypocotyl growth

Lan，Hongxia1; Heng，Yueqin1; Li，Jian1; Zhang，Mengdi1; Bian，Yeting2; Chu，Li2; Jiang，Yan1; Wang，Xuncheng3; Xu，Dongqing2; Deng，Xing Wang1,4

2022

10.1111/jipb.13350

Journal of Integrative Plant Biology   Impact Factor & Quartile

1672-9072

1744-7909

Light signaling precisely controls photomorphogenic development in plants. PHYTOCHROME INTERACTING FACTOR 4 and 5 (PIF4 and PIF5) play critical roles in the regulation of this developmental process. In this study, we report CONSTITUTIVELY PHOTOMORPHOGENIC 1 SUPPRESSOR 6 (CSU6) functions as a key regulator of light signaling. Loss of CSU6 function largely rescues the cop1-6 constitutively photomorphogenic phenotype. CSU6 promotes hypocotyl growth in the dark, but inhibits hypocotyl elongation in the light. CSU6 not only associates with the promoter regions of PIF4 and PIF5 to inhibit their expression in the morning, but also directly interacts with both PIF4 and PIF5 to repress their transcriptional activation activity. CSU6 negatively controls a group of PIF4- and PIF5-regulated gene expressions. Mutations in PIF4 and/or PIF5 are epistatic to the loss of CSU6, suggesting that CSU6 acts upstream of PIF4 and PIF5. Taken together, CSU6 promotes light-inhibited hypocotyl elongation by negatively regulating PIF4 and PIF5 transcription and biochemical activity.

COP1 CSU6 light signaling photomorphogenesis PIF4 PIF5

SCI

English

First ; Corresponding

National Natural Science Foundation of China["32100199","31900210","31970258"] ; Jiangsu Natural Science Foundation for Distinguished Young Scholars[BK20211525]

Biochemistry & Molecular Biology ; Plant Sciences

Biochemistry & Molecular Biology ; Plant Sciences

WOS:000859045200001

WILEY

2-s2.0-85138655307

Scopus

1.Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes,Department of Biology,Institute of Plant and Food Sciences,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China
2.State Key Laboratory of Crop Genetics and Germplasm Enhancement,National Center for Soybean Improvement,College of Agriculture,Nanjing Agricultural University,Nanjing,210095,China
3.Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China,Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing,100097,China
4.State Key Laboratory of Protein and Plant Gene Research,Peking-Tsinghua Center for Life Sciences,School of Advanced Agriculture Sciences and School of Life Sciences,Peking University,Beijing,100871,China

Lan，Hongxia,Heng，Yueqin,Li，Jian,et al. COP1 SUPPRESSOR 6 represses the PIF4 and PIF5 action to promote light-inhibited hypocotyl growth[J]. Journal of Integrative Plant Biology,2022.

Lan，Hongxia.,Heng，Yueqin.,Li，Jian.,Zhang，Mengdi.,Bian，Yeting.,...&Deng，Xing Wang.(2022).COP1 SUPPRESSOR 6 represses the PIF4 and PIF5 action to promote light-inhibited hypocotyl growth.Journal of Integrative Plant Biology.

Lan，Hongxia,et al."COP1 SUPPRESSOR 6 represses the PIF4 and PIF5 action to promote light-inhibited hypocotyl growth".Journal of Integrative Plant Biology (2022).