中文版 | English
Title

Transcriptomic analysis of bisphenol AF on early growth and development of zebrafish (Danio rerio) larvae

Author
Corresponding AuthorQiu,Wenhui; Zheng,Chunmiao
Publication Years
2020-10-01
DOI
Source Title
EISSN
2666-4984
Volume4
Abstract
Bisphenol AF (BPAF), an alternative to bisphenol A, is widely detected in aquatic environments. Owing to health concerns, the toxic effects of BPAF on organisms are drawing attention. The present study aims to evaluate the toxicity of BPAF, combining the results of omics techniques and experiment. Employing transcriptome sequencing (RNA-seq), we obtained 391, 648, 512, and 545 differentially expressed genes (DEGs) in 0.1, 1, 10, and 100 μg/L BPAF-exposed zebrafish larvae, respectively. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed the early development, stimulus-response, and MAPK signaling pathway were significantly affected by BPAF. In addition, five hub genes (fgf3, fgf4, map2k1, myca, and casp3b) were highlighted as the key genes in MAPK signaling pathway using the protein-protein interaction network. Therefore, the RNA-seq results showed that early development and stimulus-response were the main processes affected by BPAF, which was consistent with our morphological and pathological results. The hatching rate of zebrafish embryos in 1 and 10 μg/L BPAF groups was significantly inhibited, and the oxidative stress indexes, including the level of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and lipid peroxidation (LPO), were significantly increased by the 100 μg/L BPAF treatment. Moreover, the activity of alkaline phosphatase (AKP) was significantly decreased in all BPAF exposure groups. In conclusion, exposure to BPAF at environmental relevant concentrations affected the early development and immune system of zebrafish larvae by modulating MAPK signaling pathway, and our results provide solid evidence for the future studies on the toxicity of bisphenols.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
First ; Corresponding
Funding Project
National Natural Science Foundation of China[21707064];
WOS Accession No
WOS:000657055400001
Scopus EID
2-s2.0-85094594386
Data Source
Scopus
Citation statistics
Cited Times [WOS]:10
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/402797
DepartmentSchool of Environmental Science and Engineering
Affiliation
1.State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control,School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
2.Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control,School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
3.School of Environmental and Chemical Engineering,Shanghai University,Shanghai,200444,China
4.Shenzhen Municipal Engineering Lab of Environmental IoT Technologies,Southern University of Science and Technology,Shenzhen,518055,China
First Author AffilicationSchool of Environmental Science and Engineering
Corresponding Author AffilicationSchool of Environmental Science and Engineering
First Author's First AffilicationSchool of Environmental Science and Engineering
Recommended Citation
GB/T 7714
Li,Rongzhen,Liu,Shuai,Qiu,Wenhui,et al. Transcriptomic analysis of bisphenol AF on early growth and development of zebrafish (Danio rerio) larvae[J]. Environmental Science and Ecotechnology,2020,4.
APA
Li,Rongzhen.,Liu,Shuai.,Qiu,Wenhui.,Yang,Feng.,Zheng,Yi.,...&Zheng,Chunmiao.(2020).Transcriptomic analysis of bisphenol AF on early growth and development of zebrafish (Danio rerio) larvae.Environmental Science and Ecotechnology,4.
MLA
Li,Rongzhen,et al."Transcriptomic analysis of bisphenol AF on early growth and development of zebrafish (Danio rerio) larvae".Environmental Science and Ecotechnology 4(2020).
Files in This Item:
There are no files associated with this item.
Related Services
Fulltext link
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Export to Excel
Export to Csv
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Li,Rongzhen]'s Articles
[Liu,Shuai]'s Articles
[Qiu,Wenhui]'s Articles
Baidu Scholar
Similar articles in Baidu Scholar
[Li,Rongzhen]'s Articles
[Liu,Shuai]'s Articles
[Qiu,Wenhui]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li,Rongzhen]'s Articles
[Liu,Shuai]'s Articles
[Qiu,Wenhui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.