| Title | Engineered Spore-Forming Bacillus as a Microbial Vessel for Long-Term DNA Data Storage |
| Author | |
| Corresponding Author | Chen, Jun; Ho, Chun Loong |
| Publication Years | 2022-09-01
|
| DOI | |
| Source Title | |
| ISSN | 2161-5063
|
| Abstract | DNA data storage technology may supersede conventional chip or magnetic data storage medium, providing long-term stability, high density, and sustainable storage. Due to its error-correcting capability, DNA data stored in living organisms exhibits high fidelity in information replication. Here we report the development of a Bacillus chassis integrated with an inducible artificially assembled bacterial chromosome to facilitate random data access. We generated three sets of data in the form of DNA sequences using a rudimentary coding system accessible by the regulatory promoter. Sporulated Bacillus harboring the genes were used for long-term storage, where viability assays of spores were subjected to harsh environmental stresses to evaluate the data storage stability. The data accuracy remained above 99% after high temperature and oxidative stress treatment, whereas UV irradiation treatment provided above 96% accuracy. The developed Bacillus chassis and artificial chromosome facilitate the long-term storage of larger datum volume by using other DNA digital encoding and decoding programs. |
| Keywords | |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | First
; Corresponding
|
| Funding Project | Ministry of Science and Technology, National Key RD Program[2018YF0902604]
; National Natural Science Foundation of China?s Research Fund for International Young Scientists[22050410270]
; Shenzhen Special Fund for Innovation and Entrepreneurship of Overseas High-level Talents Peacock Team[KQTD20170810111314625]
; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191]
|
| WOS Research Area | Biochemistry & Molecular Biology
|
| WOS Subject | Biochemical Research Methods
|
| WOS Accession No | WOS:000861617900001
|
| Publisher | |
| Data Source | Web of Science
|
| Citation statistics |
Cited Times [WOS]:2
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/406032 |
| Department | Department of Biomedical Engineering |
| Affiliation | 1.Southern Univ Sci & Technol SUSTech, Dept Biomed Engn, Shenzhen 518055, Peoples R China 2.Chinese Acad Sci, Shenzhen Inst Adv Technol SIAT, Shenzhen Inst Synthet Biol, Shenzhen 518055, Peoples R China |
| First Author Affilication | Department of Biomedical Engineering |
| Corresponding Author Affilication | Department of Biomedical Engineering |
| First Author's First Affilication | Department of Biomedical Engineering |
| Recommended Citation GB/T 7714 |
Liu, Feng,Li, Jiashu,Zhang, Tongzhou,et al. Engineered Spore-Forming Bacillus as a Microbial Vessel for Long-Term DNA Data Storage[J]. ACS Synthetic Biology,2022.
|
| APA |
Liu, Feng,Li, Jiashu,Zhang, Tongzhou,Chen, Jun,&Ho, Chun Loong.(2022).Engineered Spore-Forming Bacillus as a Microbial Vessel for Long-Term DNA Data Storage.ACS Synthetic Biology.
|
| MLA |
Liu, Feng,et al."Engineered Spore-Forming Bacillus as a Microbial Vessel for Long-Term DNA Data Storage".ACS Synthetic Biology (2022).
|
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